Detection of Latent DNA Using a DNA Binding Dye.

Area of Science:

• Forensic Science
• Molecular Biology
• Biochemistry

Background:

• Latent DNA, or
• touch DNA
• , is deposited on items through contact and is vital forensic evidence.
• Previously, visualizing latent DNA was not possible.
• The development of DNA-binding dyes has enabled latent DNA visualization.

Purpose of the Study:

• To detail the process of detecting latent DNA using Diamond™ Nucleic Acid Dye.
• To demonstrate the dye's utility on both control and evidential items.
• To enable a targeted approach to crime scene collection through DNA cell location identification.

Main Methods:

• Application of Diamond™ Nucleic Acid Dye in ethanol or water solution.
• Visualization of stained cellular material using a mobile microscope at 50X and 220X magnification.
• Photography of stained cells to document DNA presence and location.

Main Results:

• The dye effectively visualizes cellular material containing latent DNA as green dots.
• Microscopy confirms the presence of cells at 220X magnification.
• Successful staining and documentation of DNA on non-absorbent surfaces.

Conclusions:

• Diamond™ Nucleic Acid Dye provides a simple, rapid method for visualizing latent touch DNA.
• This technique enhances forensic capabilities by allowing for targeted evidence collection.
• The method is effective on non-absorbent surfaces and aids in documenting DNA evidence.