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Related Concept Videos

RNA-seq03:21

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Updated: Jul 23, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
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Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

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Nanopore sequencing technology and its applications.

Peijie Zheng1, Chuntao Zhou1, Yuemin Ding1,2,3

  • 1Department of Clinical Medicine School of Medicine Zhejiang University City College Hangzhou China.

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|July 13, 2023
PubMed
Summary
This summary is machine-generated.

Nanopore sequencing, a third-generation technology, offers long reads and portability for diverse applications. Innovations have improved its accuracy, making it vital for pandemic response and future research in genomics and beyond.

Keywords:
COVID‐19SARS‐CoV‐2cancergenomemutationnanopore sequencingpandemicplant

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Area of Science:

  • Genomics and Molecular Biology
  • Bioinformatics
  • Epidemiology

Background:

  • Sanger sequencing revolutionized genetic code interpretation since 1977.
  • Nanopore sequencing is a leading third-generation technology known for long reads, portability, and cost-effectiveness.
  • Initial concerns regarding nanopore sequencing's accuracy have been mitigated by technological advancements.

Purpose of the Study:

  • To highlight the critical role of nanopore sequencing in detecting SARS-CoV-2 during the COVID-19 pandemic.
  • To promote understanding and adoption of nanopore sequencing for emerging outbreaks and other research areas.
  • To discuss the applications and strategies for improving nanopore sequencing accuracy.

Main Methods:

  • Review of nanopore sequencing technology and its applications.
  • Analysis of nanopore sequencing's contribution to COVID-19 detection and control.
  • Exploration of applications in microbial, cancer, and plant genomics.

Main Results:

  • Nanopore sequencing was instrumental in identifying the SARS-CoV-2 genome and aiding pandemic containment.
  • Continuous innovation has significantly enhanced the accuracy of nanopore sequencing platforms and algorithms.
  • The technology demonstrates broad utility across epidemic prevention, disease diagnosis, and breeding programs.

Conclusions:

  • Nanopore sequencing is becoming a mainstream tool for epidemic control and has significant potential in oncology and botany.
  • Increased understanding of nanopore sequencing will drive its wider application in global health and research.
  • Further improvements in accuracy will solidify nanopore sequencing's position in various genomic applications.