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Bioelectrochemical system accelerates reductive dechlorination through extracellular electron transfer networks.

Su-Hao Chen1, Zheng-Tao Li1, He-Ping Zhao1

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Electrostimulation in bioelectrochemical systems significantly enhances trichloroethylene bio-dechlorination by promoting electron transfer. This study clarifies the mechanisms, revealing how electrical stimulation enriches key microbial populations for improved contaminant removal.

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Area of Science:

  • Environmental Microbiology
  • Electrochemistry
  • Bioremediation

Background:

  • Bioelectrochemical systems (BES) offer a promising avenue for enhanced bio-dechlorination.
  • The precise mechanisms of extracellular electron transfer in dechlorinating microbial consortia remain incompletely understood.

Purpose of the Study:

  • To investigate the effectiveness of electrostimulation in enhancing trichloroethylene (TCE) reduction within a bioelectrochemical system.
  • To elucidate the underlying mechanisms of electron transfer and microbial community dynamics during electrostimulated bio-dechlorination.

Main Methods:

  • Establishment of bioelectrochemical systems with controlled cathode potential (-0.30 V vs. SHE).
  • Measurement of dechlorination rates and electrochemical activity using cyclic voltammetry.
  • Analysis of bacterial community structure, metagenomics, and quantitative PCR (qPCR).

Main Results:

  • Electrostimulated biocathodes achieved a 1.36 times higher average dechlorination rate (102.0 μM Cl·d⁻¹) compared to open-circuit controls (74.7 μM Cl·d⁻¹).
  • Electrostimulation boosted electrochemical activity and facilitated the enrichment of electroactive and dechlorinating bacteria on the cathode.
  • Metagenomic and qPCR analyses identified direct electron transfer pathways (e.g., conductive pili, cytochromes) and indirect pathways (e.g., riboflavin) contributing to enhanced dechlorination.

Conclusions:

  • Electrostimulated bio-dechlorination in BES is highly effective for TCE reduction.
  • Electrical stimulation optimizes microbial consortia and electron transfer mechanisms for improved bioremediation efficiency.
  • This study provides critical insights into the electron transfer networks driving enhanced dechlorination in BES.