Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genetic Screens02:46

Genetic Screens

5.0K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
5.0K
Experimental RNAi02:15

Experimental RNAi

6.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The genetics and mechanisms of parasitoid host specificity.

Current opinion in insect science·2026
Same author

Genome divergence and reproductive incompatibility among populations of Ganaspis near brasiliensis.

G3 (Bethesda, Md.)·2024
Same author

Cold tolerance and overwintering survival of <i>Aphelinus certus</i> (Hymenoptera: Aphelinidae), a parasitoid of the soybean aphid (Hemiptera: Aphididae) in North America.

Bulletin of entomological research·2023
Same author

Assemblies of the genomes of parasitic wasps using meta-assembly and scaffolding with genetic linkage.

G3 (Bethesda, Md.)·2021
Same author

Response to selection for parasitism of a suboptimal, low-preference host in an aphid parasitoid.

Evolutionary applications·2021
Same author

Immune Suppressive Extracellular Vesicle Proteins of <i>Leptopilina heterotoma</i> Are Encoded in the Wasp Genome.

G3 (Bethesda, Md.)·2019

Related Experiment Video

Updated: Jul 23, 2025

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points
08:55

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

Published on: May 29, 2020

7.8K

Reduced-representation libraries in insect genetics.

Keith R Hopper1

  • 1Beneficial Insect Introductions Research Unit, ARS, USDA, Newark, DE, United States.

Current Opinion in Insect Science
|July 13, 2023
PubMed
Summary

Genotyping-by-sequencing (GBS) enables genome-wide data for any species. Recent studies show GBS reveals population differences, local adaptation, and genetic divergence across diverse taxa.

Area of Science:

  • Genomics
  • Population Genetics
  • Bioinformatics

Background:

  • Genotyping-by-sequencing (GBS) of reduced-representation libraries provides genome-wide data across diverse species.
  • Recent advancements have expanded its application in evolutionary and ecological research.

Approach:

  • A comprehensive literature review of GBS research from 2021-2022 was conducted.
  • Meta-analysis of findings from 21 studies across 33 species was performed.
  • Analysis methods and challenges associated with GBS data were discussed.

Key Points:

  • The majority of reviewed studies focused on population differences, local adaptation, and migration.
  • Applications included genetic mapping, assembly scaffolding, quantitative trait loci (QTL) analysis, pest incursions, and pathogen infection rates.

More Related Videos

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
09:57

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Published on: December 28, 2016

10.7K
Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes
18:38

Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes

Published on: September 25, 2013

12.1K

Related Experiment Videos

Last Updated: Jul 23, 2025

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points
08:55

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

Published on: May 29, 2020

7.8K
Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
09:57

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Published on: December 28, 2016

10.7K
Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes
18:38

Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes

Published on: September 25, 2013

12.1K
  • Meta-analysis indicated that local adaptation typically involves a small fraction (<16%, often <1%) of the genome.

    • Conclusions:

    • The number of adaptive loci identified through GBS correlates with genetic divergence between populations.
    • GBS is a powerful tool for understanding genome-wide patterns of adaptation and population structure in various species.