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Post-Translational Modification β-Hydroxybutyrylation Regulates Ustilaginoidea virens Virulence
Xiaoyang Chen1, Yuhang Duan2, Zhiyong Ren3
1Anhui Province Key Laboratory of Crop Integrated Pest Management, Anhui Agricultural University, Hefei, China.
Molecular & Cellular Proteomics : MCP
|July 13, 2023
Summary
Lysine β-hydroxybutyrylation (Kbhb) regulates fungal virulence in Ustilaginoidea virens. This post-translational modification impacts protein activity and localization, offering insights into fungal pathogen control.
Area of Science:
- Biochemistry
- Molecular Biology
- Plant Pathology
Background:
- Lysine β-hydroxybutyrylation (Kbhb) is a widespread post-translational modification linked to gene transcription and cell proliferation.
- The function of Kbhb in phytopathogenic fungi, particularly in plant diseases, is largely unexplored.
Purpose of the Study:
- To investigate the role and regulatory mechanisms of lysine β-hydroxybutyrylation (Kbhb) in the phytopathogenic fungus Ustilaginoidea virens.
- To identify Kbhb sites and proteins involved in fungal virulence and cellular processes.
Main Methods:
- Proteomic analysis to identify Kbhb sites and proteins in Ustilaginoidea virens.
- Site-directed mutagenesis to study the functional impact of Kbhb on specific proteins (UvSlt2, UvCdc10).
- Molecular dynamic simulations to understand the structural effects of Kbhb.
- Enzyme assays to identify Kbhb-removing enzymes (histone deacetylases).
Main Results:
- 2204 Kbhb sites were identified across 852 proteins in U. virens.
- Mutation of Kbhb sites in UvSlt2 and UvCdc10 led to reduced kinase activity, altered protein localization, and decreased fungal virulence.
- NAD+-dependent histone deacetylases UvSirt2 and UvSirt5 were identified as key enzymes responsible for Kbhb removal.
Conclusions:
- Lysine β-hydroxybutyrylation plays a critical role in regulating protein function, localization, and enzymatic activity in U. virens.
- Kbhb is a significant post-translational modification influencing the virulence of phytopathogenic fungi.
- Understanding the Kbhb pathway provides novel targets for controlling fungal plant diseases.
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