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Pediatric reference intervals for hematology parameters in healthy infants and young children in Iran

Maliheh Mohammadi1, Hamideh Ghazizadeh2,3, Maryam Mohammadi-Bajgiran3

  • 1Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.

International Journal of Laboratory Hematology
|July 13, 2023
PubMed

Insights

Accurate pediatric hematology reference intervals (RIs) were established for 11 parameters in Iranian children. Six parameters showed significant age-related changes, highlighting the need for pediatric-specific standards.

Area of Science:

  • Pediatric Hematology
  • Clinical Diagnostics
  • Reference Interval Establishment

Background:

  • Accurate age- and sex-specific reference intervals (RIs) are crucial for pediatric clinical diagnosis.
  • Gaps exist in current RIs for hematologic parameters in young children.
  • This study aimed to establish age-specific RIs for 11 hematologic parameters in Iranian children.

Purpose of the Study:

  • To determine age-specific reference intervals for 11 hematologic parameters in Iranian children.
  • To identify which hematologic parameters require age-based partitioning in pediatrics.
  • To provide updated reference standards for pediatric hematology.

Main Methods:

  • Collected blood samples from 344 Iranian children aged 3 days to 30 months.
  • Analyzed 11 complete blood count (CBC) parameters using a Sysmex auto-analyzer.
  • Calculated RIs with 90% confidence intervals using the direct method.

Main Results:

  • No sex-specific partitioning was required for CBC parameters.
  • Six of 11 CBC parameters demonstrated significant age-related changes, necessitating age-based RIs.
  • Parameters like red blood cell count and hemoglobin increased with age, while mean corpuscular volume and platelet count decreased.

Conclusions:

  • Established age-specific RIs for 11 hematologic parameters in young Iranian children.
  • Confirmed the necessity of age partitioning for six hematologic parameters due to significant developmental changes.
  • Emphasized the importance of pediatric-specific reference standards for accurate diagnosis and monitoring.
Abstract

Keywords:
CBC parametersCLSI Ep28-A3 guidelineshematologypediatricreference intervals

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