Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 23, 2025

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
12:31

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization

Published on: March 25, 2009

23.4K

Label-free adaptive optics single-molecule localization microscopy for whole zebrafish.

Sanghyeon Park1,2, Yonghyeon Jo1,2, Minsu Kang3

  • 1Center for Molecular Spectroscopy and Dynamics, Institute for Basic Science, Seoul, Republic of Korea.

Nature Communications
|July 13, 2023
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Thrombosis in adult primary immune thrombocytopenia in Korea: incidence and risk factors in a 20-year retrospective cohort.

Annals of hematology·2026
Same author

Lenvatinib plus pembrolizumab versus standard of care in recurrent/metastatic head and neck squamous cell carcinoma: Randomized, phase 2 LEAP-009 trial.

European journal of cancer (Oxford, England : 1990)·2026
Same author

TiON/NiO<i><sub>x</sub></i> Heterojunction Neuron for CMOS-Compatible Hardware Implementation of Activation Function in Neural Network.

ACS applied materials & interfaces·2026
Same author

Experimental structural characterization of reflection matrices of scattering media.

Biomedical optics express·2026
Same author

Functional Ureteral Obstruction Due to Retroperitoneal Tissue Interposition During Oblique Lumbar Interbody Fusion: A Report of Two Cases.

Journal of clinical medicine·2026
Same author

Asymptomatic tuberculosis detected in health screening predicts favourable outcome.

ERJ open research·2026

This study introduces label-free adaptive optics for deep-tissue super-resolution imaging using single-molecule localization microscopy (SMLM). It overcomes specimen-induced aberrations, enabling clearer imaging deeper within biological tissues.

Area of Science:

  • Biomedical Optics
  • Microscopy
  • Neuroscience

Background:

  • Specimen-induced aberrations limit imaging depth in single-molecule localization microscopy (SMLM).
  • Overcoming these aberrations is crucial for deep-tissue super-resolution imaging.

Purpose of the Study:

  • To apply label-free wavefront sensing adaptive optics to SMLM for enhanced deep-tissue super-resolution imaging.
  • To overcome the limitations of specimen-induced aberrations in SMLM.

Main Methods:

  • Developed a label-free adaptive optics system for SMLM.
  • Measured complex tissue aberrations using intrinsic reflectance, not fluorescence.
  • Physically corrected wavefront distortions exceeding previous limits.

Main Results:

More Related Videos

Single Cell Fate Mapping in Zebrafish
07:53

Single Cell Fate Mapping in Zebrafish

Published on: October 5, 2011

13.5K
Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish
07:28

Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish

Published on: February 27, 2014

19.0K

Related Experiment Videos

Last Updated: Jul 23, 2025

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
12:31

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization

Published on: March 25, 2009

23.4K
Single Cell Fate Mapping in Zebrafish
07:53

Single Cell Fate Mapping in Zebrafish

Published on: October 5, 2011

13.5K
Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish
07:28

Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish

Published on: February 27, 2014

19.0K
  • Achieved super-resolution imaging up to 102 μm deep in whole zebrafish and mouse brain tissues.
  • Resolved sub-diffraction morphologies of cilia, oligodendrocytes, and dendritic spines.
  • Enhanced localization number by up to 37 times with precision comparable to aberration-free samples.

Conclusions:

  • Label-free adaptive optics significantly expands SMLM's application range for deep-tissue imaging.
  • The system effectively corrects severe tissue aberrations, preventing loss of localization number.
  • Enables high-resolution imaging in previously inaccessible biological samples like whole zebrafish.