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Induced regional differences in cutaneous cell-mediated immunity in the mouse.
International Archives of Allergy and Applied Immunology
|January 1, 1986
Summary
Mice exposed to an antigen on one ear developed strong local immunity but suppressed immunity in the other ear. This contralateral suppression, mediated by suppressor cells, lasted for weeks and could be blocked by cyclophosphamide.
Area of Science:
- Immunology
- Dermatology
Background:
- Cell-mediated immunity (CMI) is crucial for immune responses.
- Regional variations in immune responses can occur.
- Suppressor cells play a role in immune regulation.
Purpose of the Study:
- To investigate the induction of regional variations in cutaneous cell-mediated immunity (CMI) in mice.
- To determine the duration and mechanism of contralateral immune unresponsiveness.
Main Methods:
- Mice were sensitized with 1-fluoro-2,4-dinitrobenzene (DNFB) or oxazolone on one ear.
- Antigenic rechallenge was performed on both the treated and contralateral ears.
- Cyclophosphamide was administered to some mice prior to sensitization.
Main Results:
- Sensitization induced a strong local CMI response in the treated ear.
- A minimal CMI response was observed in the contralateral ear, persisting for up to 14 weeks.
- Cyclophosphamide treatment before sensitization abolished the contralateral suppression.
Conclusions:
- Mice exhibit induced regional variations in cutaneous CMI intensity.
- Contralateral ear unresponsiveness is mediated by suppressor cells.
- These findings highlight the plasticity of the immune system and potential for immune modulation.