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A Mannose Receptor from Litopenaeus vannamei Involved in Innate Immunity by Pathogen Recognition and Inflammation

Na Guo1,2, Yuan Liu2,3, Qiang Hao1,2

  • 1School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China.

International Journal of Molecular Sciences
|July 14, 2023
PubMed

Insights

The Pacific white shrimp mannose receptor (LvMR) recognizes pathogens and binds sugars. LvMR acts as a negative regulator of inflammation and reactive oxygen species during bacterial infection.

Area of Science:

  • Immunology
  • Marine Biology
  • Molecular Biology

Background:

  • Mannose receptors (MRs) are C-type lectin superfamily members involved in pattern recognition and cellular signaling.
  • The Pacific white shrimp (Litopenaeus vannamei) is a commercially important aquaculture species facing significant disease challenges.

Purpose of the Study:

  • To identify and characterize a mannose receptor gene (LvMR) in Litopenaeus vannamei.
  • To investigate the function of LvMR in immune recognition and inflammatory responses in shrimp.

Main Methods:

  • Gene identification and sequence analysis of LvMR.
  • Recombinant protein expression and binding assays with PAMPs and carbohydrates.
  • Gene knockdown experiments using RNA interference.
  • Analysis of immune gene expression, ROS content, and iNOS activity post-infection.

Main Results:

  • LvMR possesses characteristic domains (FN II, CRDs) and motifs (EPS, FND).
  • LvMR transcripts are primarily in the hepatopancreas and respond to pathogen challenge.
  • Recombinant LvMR binds PAMPs and agglutinates microbes in a Ca2+-dependent manner, with high affinity for D-mannose.
  • LvMR knockdown increases NF-κB pathway genes, inflammation, and redox genes, but not phagocytosis genes.
  • LvMR knockdown elevates ROS and iNOS activity following Vibrio parahaemolyticus infection.

Conclusions:

  • LvMR functions as a pattern recognition receptor (PRR) in shrimp immune recognition.
  • LvMR acts as a negative regulator of inflammatory responses during bacterial infection in shrimp.
Keywords:
Litopenaeus vannameiROSinflammationmannose receptorpathogen recognition

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