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MicroRNA PC-3p-2869 Regulates Antler Growth and Inhibits Proliferation and Migration of Human Osteosarcoma and

Fan Yang1, Jin Wu1, Mindie Zhao1

  • 1Laboratory of Genetics and Molecular Biology, College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, China.

International Journal of Molecular Sciences
|July 14, 2023
PubMed

Insights

A novel microRNA (miRNA) from deer antlers, miR-PC-2869, suppresses bone cancer cell growth. This discovery highlights its potential as a new therapeutic for osteosarcoma and chondrosarcoma.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Regenerative Medicine

Background:

  • MicroRNAs (miRNAs) are critical regulators of cellular processes, including tumorigenesis and tissue regeneration.
  • Antler regeneration involves a complex interplay between cell growth and suppression, with miRNAs playing a key role.
  • The therapeutic potential of naturally occurring molecules in regenerative processes for cancer treatment is an area of active investigation.

Purpose of the Study:

  • To investigate the role of a novel antler-derived microRNA, PC-3p-2869 (miR-PC-2869), in antler growth regulation.
  • To explore the therapeutic potential of miR-PC-2869 in human osteosarcoma and chondrosarcoma cell lines.
  • To elucidate the molecular mechanism underlying miR-PC-2869's function and its target genes.

Main Methods:

  • Stem-loop RT-qPCR was used to detect miR-PC-2869 expression in antler tissues.
  • Overexpression and knockdown experiments were performed in antler cartilage cells and human bone cancer cell lines (MG63, U2OS, SW1353).
  • Reporter assays were employed to identify functional target genes of miR-PC-2869, followed by expression analysis and mechanistic studies involving c-myc and cyclin D1.

Main Results:

  • miR-PC-2869 was widely expressed in antler tissues and suppressed the proliferation and migration of antler cartilage cells.
  • Heterologous expression of miR-PC-2869 significantly reduced proliferation, colony formation, and migration in osteosarcoma and chondrosarcoma cell lines.
  • miR-PC-2869 targets conserved genes (e.g., CDK8, EEF1A1, NTN1) that mediate the regulation of c-myc and cyclin D1, explaining its anti-cancer effects.

Conclusions:

  • Antler-derived miR-PC-2869 functions as a tumor suppressor by inhibiting cell proliferation and migration.
  • The identified target genes CDK8, EEF1A1, and NTN1 are crucial mediators of miR-PC-2869's anti-cancer activity.
  • miR-PC-2869 represents a promising therapeutic candidate for human osteosarcoma and chondrosarcoma.
Keywords:
antlercell proliferationchondrosarcomacross-species regulationmicroRNA PC-3p-2869osteosarcoma

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