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A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3
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TRIM21 Promotes Rabies Virus Production by Degrading IRF7 through Ubiquitination
Boyue Zhang1, Ting Cai1, Hongling He1
1College of Veterinary Medicine, South China Agricultural University, Guangzhou 510000, China.
International Journal of Molecular Sciences
|July 14, 2023
Summary
Tripartite-motif protein 21 (TRIM21) is essential for rabies virus replication. Targeting TRIM21 may offer a new strategy for treating rabies, a fatal zoonotic disease.
Area of Science:
- Virology
- Immunology
- Molecular Biology
Background:
- Rabies is a fatal zoonotic disease with unknown pathogenic mechanisms and no effective treatments.
- The tripartite-motif protein (TRIM) family regulates viral replication.
- Tripartite-motif protein 21 (TRIM21) expression is upregulated in rabies virus-infected mouse brains.
Purpose of the Study:
- To investigate the role of TRIM21 in rabies virus replication.
- To elucidate the mechanism by which TRIM21 affects rabies virus replication.
- To identify TRIM21 as a potential therapeutic target for rabies.
Main Methods:
- Analyzing TRIM21 expression in rabies virus-infected mouse brains.
- Investigating the effect of TRIM21 knockdown and overexpression on rabies virus replication.
- Examining the role of interferon-alpha, interferon-beta, and interferon regulatory factor 7 (IRF7) in TRIM21-mediated regulation of rabies virus replication.
Main Results:
- TRIM21 knockdown inhibited rabies virus replication, while overexpression promoted it.
- TRIM21 regulates rabies virus replication through the IRF7-interferon axis.
- TRIM21 was identified as a novel host factor essential for rabies virus replication.
Conclusions:
- TRIM21 is a critical host factor for rabies virus replication.
- TRIM21 represents a potential therapeutic target for rabies treatment and management.
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