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3D Cultures for Modelling the Microenvironment: Current Research Trends and Applications

Roberto Gaetani1, Isotta Chimenti2,3

  • 1Department of Molecular Medicine, Faculty of Pharmacy and Medicine, Sapienza University of Rome, 00185 Rome, Italy.

International Journal of Molecular Sciences
|July 14, 2023
PubMed

Abstract:

The importance of 3D culture systems for drug screening or physio-pathological models has exponentially increased in recent years [...].

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Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
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