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Published on: May 28, 2012
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Study on Extraction, Physicochemical Properties, and Bacterio-Static Activity of Polysaccharides from Phellinus
Nengbin Gao1, Weijia Zhang1, Dianjie Hu1
1College of Food Science and Engineering, Changchun University, Changchun 130012, China.
Molecules (Basel, Switzerland)
|July 14, 2023
Summary
Optimized ultrasound-assisted extraction of Phellinus linteus mycelium polysaccharides (PLPs) yielded 12.98%. These PLPs, rich in glucose, mannose, galactose, and glucuronic acid, demonstrated significant bacteriostatic properties against common bacteria.
Area of Science:
- Mycology and Biochemistry
- Natural Product Chemistry
Background:
- Phellinus linteus mycelium polysaccharides (PLPs) are known for their potential bioactivities.
- Optimizing extraction methods is crucial for maximizing yield and purity of bioactive compounds.
Purpose of the Study:
- To optimize the ultrasound-assisted extraction of Phellinus linteus mycelium polysaccharides (PLPs).
- To analyze the monosaccharide composition of the extracted PLPs.
- To evaluate the bacteriostatic properties of PLPs against selected bacterial strains.
Main Methods:
- Ultrasound-assisted extraction optimized using a Box-Behnken design.
- Chemical analysis for composition and High-Performance Liquid Chromatography (HPLC) for monosaccharide profiling.
- Spectrophotometric turbidimetric method for assessing bacteriostatic activity.
- Scanning electron microscopy for morphological analysis.
Main Results:
- Optimal extraction conditions: 60°C, 20 min, 1:42 g/mL solvent ratio, yielding 12.98% PLPs.
- PLPs primarily composed of glucose, mannose, galactose, and glucuronic acid.
- Intracellular polysaccharides (PIP) showed stronger inhibition against S. aureus and E. coli than extracellular polysaccharides (PEP).
Conclusions:
- Ultrasound-assisted extraction is an effective method for obtaining Phellinus linteus mycelium polysaccharides.
- The extracted PLPs possess significant bacteriostatic potential, with variations observed between intracellular and extracellular fractions.
- Further research into the specific applications of these polysaccharides in antimicrobial therapies is warranted.
