Related Experiment Video
Updated: Jul 23, 2025
08:45
Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity
Published on: September 7, 2011
52.9K
Tannins Can Have Direct Interactions with Anthelmintics: Investigations by Isothermal Titration Calorimetry
Mimosa Sillanpää1, Marica T Engström1, Petri Tähtinen1
1Department of Chemistry, University of Turku, FI-20014 Turku, Finland.
Molecules (Basel, Switzerland)
|July 14, 2023
Summary
Plant tannins show direct interactions with thiabendazole, an anthelmintic. Their combined use may affect drug efficacy, with structural features influencing the interaction strength.
Area of Science:
- Veterinary Pharmacology
- Natural Product Chemistry
- Parasitology
Background:
- Plant tannins possess anthelmintic and antiparasitic properties, making them a focus for combating anthelmintic resistance.
- Investigating tannins as complementary agents with commercial anthelmintics is a promising strategy, though interactions are not fully understood.
- Limited research exists on tannin-anthelmintic interactions, with reported synergistic and antagonistic effects influencing drug efficacy.
Purpose of the Study:
- To investigate the direct interactions between hydrolysable tannins (HTs) and the benzimidazole anthelmintic thiabendazole (TBZ).
- To understand how structural features of HTs influence their interaction with TBZ.
- To assess the potential implications of these interactions on the efficacy of commercial anthelmintics.
Main Methods:
- Selection of a series of hydrolysable tannins (HTs) for study.
- Evaluation of direct interactions between selected HTs and thiabendazole (TBZ) using isothermal titration calorimetry (ITC).
- Analysis of structural features of HTs contributing to the observed interactions.
Main Results:
- Hydrolysable tannins (HTs) exhibit direct exothermic interactions with thiabendazole (TBZ).
- The strength of the HT-TBZ interaction is positively correlated with the number of free galloyl groups in the tannin structure.
- Increased molecular flexibility of HTs also enhances their interaction with TBZ.
Conclusions:
- Hydrolysable tannins (HTs) can directly interact with the benzimidazole anthelmintic thiabendazole (TBZ).
- The structural characteristics of HTs, specifically galloyl group abundance and molecular flexibility, significantly modulate these interactions.
- Findings provide insights into the complex interplay between plant-derived compounds and commercial anthelmintics, relevant for managing anthelmintic resistance.
Related Concept Videos
01:23EDTA: Indirect and Alkalimetric Titration
998
Unlike direct titration, back-titration, and displacement titration, indirect titration is an EDTA titration method for quantifying anions. In the indirect titration method, anions are precipitated as their insoluble salts with excess metal ions. The filtrate containing the excess metal ions is directly titrated with standard EDTA until the endpoint is achieved. Another approach involves extracting the metal ion and back-titrating with standard EDTA to obtain the endpoint. In this way, the...
998
01:01Classification of Titrimetric Analysis Based on Reaction Types
786
Titrimetric analysis in solution chemistry involves measuring the volume of solutions and is often called volumetric analysis. The standard solution of known concentration in the burette is called the titrant, whereas the solution of unknown concentration in the flask is called the analyte, or titrand. Titrimetric analyses can be classified into four types based on the reactions between the titrant and analyte.
Titrations between an acid and a base lead to neutralization reactions that form...
Titrations between an acid and a base lead to neutralization reactions that form...
786
01:26EDTA: Auxiliary Complexing Reagents
623
EDTA titrations are usually carried out in highly basic conditions, where the fully deprotonated form of EDTA, Y4−, actively complexes with the free metal ions in the solution. Several metal ions precipitate as hydrous oxide (hydroxides, oxides, or oxyhydroxides) under these conditions, lowering the concentration of free metal ions in the solution. For this reason, auxiliary complexing agents or ligands such as ammonia, tartrate, citrate, or triethanolamine are used in EDTA titrations to...
623
01:08Titrimetric Methods: Types and Commonly Used Strategies
987
In chemistry, titrimetric methods are broadly classified into three types: volumetric, gravimetric, and coulometric. Volumetric titrations involve measuring the volume of a titrant of known concentration that is required to react completely with an analyte. In gravimetric titrations, the standard solution reacts with the analyte to form an insoluble precipitate, which is filtered, dried, and weighed. In coulometric titrations, current is applied to an electrochemical reaction until the reaction...
987
00:39Complexometric Titration: Overview
7.3K
Complexometric titration involves the formation of a complex by reacting a metal ion with one or more ligands. A visual indicator often detects the end point of a complexometric titration. It is added to the metal solution before the titration, forming a stable metal–indicator complex and imparting color to the solution. As the titration approaches the equivalence point, the excess of the added ligand displaces the indicator from the metal–indicator complex, releasing the free...
7.3K
00:43Complexometric Titration: Ligands
1.0K
Different monodentate and polydentate ligands are used as complexing agents in complexometric titration reactions. The formation of complexes by mono- and bidentate ligands involves two or more intermediate steps, limiting their use as complexing agents. In comparison, polydentate ligands can form complexes with metal ions in a single-step process, facilitating sharper end points. This means polydentate ligands, such as amino carboxylic acid derivatives, are most commonly employed in...
1.0K