The Sweet Potato K⁺ Transporter IbHAK11 Regulates K⁺ Deficiency and High Salinity Stress Tolerance by Maintaining...

The K⁺ transporter KT/HAK/KUP (K⁺ transporter/high-affinity K⁺/K⁺ uptake) family has a critical effect on K⁺ uptake and translocation in plants under different environmental conditions. However, the functional analysis of KT/HAK/KUP members in sweet potatoes is still limited. The present work reported the physiological activity of a new gene, IbHAK11, in the KT/HAK/KUP family in sweet potatoes. IbHAK11 expression increased significantly in the low K⁺-tolerant line compared with the low K⁺-sensitive line following treatment with low K⁺ concentrations. IbHAK11 upregulation promoted root growth in Arabidopsis under low K⁺ conditions. Under high saline stress, transgenic lines had superior growth and photosynthetic characteristics compared with the wild-type (WT). As for IbHAK11-overexpressing plants, activation of both the non-enzymatic and enzymatic reactive oxygen species (ROS) scavenging systems was observed. Therefore, IbHAK11-overexpressing plants had lower malondialdehyde (MDA) and ROS levels (including H₂O₂ and O^2-) compared with WT under salt-induced stress. We also found that under both low K⁺ and high salinity conditions, overexpression of IbHAK11 enhanced K⁺ translocation from the root to the shoot and decreased Na⁺ absorption in Arabidopsis. Consequently, IbHAK11 positively regulated K⁺ deficiency and high salinity stresses by regulating K⁺ translocation and Na⁺ uptake, thus maintaining K⁺/Na⁺ homeostasis in plants.