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Updated: Jul 23, 2025
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Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye
Published on: June 20, 2019
7.8K
Stable wide-field voltage imaging for observing neuronal plasticity at the neuronal network level
Takashi Tominaga1,2, Riichi Kajiwara3, Yoko Tominaga1
1Institute of Neuroscience, Tokushima Bunri University, Sanuki, Kagawa 769-2193, Japan.
Biophysics and Physicobiology
|July 14, 2023
Summary
This review explores how voltage-sensitive dyes (VSDs) visualize brain plasticity, crucial for learning and memory. It details VSDs
Area of Science:
- Neuroscience
- Neurophysiology
- Synaptic Plasticity
Background:
- Brain plasticity, particularly in hippocampal synapses, is fundamental for learning and memory.
- Changes in neuronal circuit function are a direct consequence of synaptic plasticity.
Purpose of the Study:
- To review the application of voltage-sensitive dyes (VSDs) for investigating neuronal circuit plasticity.
- To discuss the significance of plastic changes in circuit function and the technical challenges associated with VSD usage.
Main Methods:
- Utilizing voltage-sensitive dyes (VSDs) for membrane potential imaging.
- Long-term recording techniques for analyzing neural circuit plasticity.
Main Results:
- VSDs enable the examination of neuronal circuit plasticity.
- Plastic changes in hippocampal circuits, influenced by long-term potentiation and entorhinal-perirhinal connections, are discussed.
Conclusions:
- Voltage-sensitive dyes are valuable tools for studying brain plasticity and its role in learning and memory.
- Further research into VSDs can enhance our understanding of neural circuit dynamics and function.
