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Related Experiment Video

Updated: Jul 23, 2025

Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation
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Enzybiotic-mediated antimicrobial functionalization of polyhydroxyalkanoates.

Francisco G Blanco1,2, Roberto Vázquez3, Ana M Hernández-Arriaga1,2

  • 1Polymer Biotechnology Group, Microbial and Plant Biotechnology Department, Margarita Salas Center for Biological Research (CIB-CSIC), Madrid, Spain.

Frontiers in Bioengineering and Biotechnology
|July 14, 2023
PubMed
Summary

Polymeric nanoparticles made from polyhydroxyalkanoates (PHAs) were functionalized with an antimicrobial enzyme to create a novel treatment for Streptococcus pneumoniae infections. This innovative nanosystem effectively targets and eliminates bacteria in both free and biofilm forms.

Keywords:
antimicrobial materialsantimicrobial nanoparticlesdrug deliveryenzybioticspolyhydroxyalkanoates

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Microbiology

Background:

  • Polymeric nanoparticles (NPs) offer advantages for drug delivery, including barrier translocation.
  • Polyhydroxyalkanoates (PHAs) are biocompatible and biodegradable biopolymers suitable for hydrophobic environments like lung surfactant.
  • Streptococcus pneumoniae causes severe respiratory infections like community-acquired pneumonia.

Purpose of the Study:

  • To develop an antimicrobial material by immobilizing an enzybiotic onto PHA nanoparticles.
  • To create a targeted delivery system for combating Streptococcus pneumoniae infections in the lungs.

Main Methods:

  • Production of a fusion protein (M711) combining a PHA affinity tag (MinP) and an enzybiotic (Cpl-711).
  • Formulation of PHA nanoparticles suitable for pulmonary delivery and decoration with M711.
  • Assessment of the nanosystem's antimicrobial activity against planktonic and biofilm Streptococcus pneumoniae.

Main Results:

  • The M711 fusion protein successfully immobilized onto PHA nanoparticles.
  • The resulting nanosystem demonstrated sustained antimicrobial activity against both free and sessile Streptococcus pneumoniae.
  • Tag-mediated immobilization provides a viable method for creating bioactive antimicrobial functionalized PHAs.

Conclusions:

  • PHA nanoparticles functionalized with enzybiotics via affinity tags represent a promising platform for antimicrobial applications.
  • This approach offers a novel strategy for developing targeted therapies against bacterial lung infections.
  • The study highlights the potential of biopolymer-based nanocarriers for advanced biomedical applications.