A distinct stimulatory cDC1 subpopulation amplifies CD8⁺ T cell responses in tumors for protective anti-cancer...

Type 1 conventional dendritic cells (cDC1) can support T cell responses within tumors but whether this determines protective versus ineffective anti-cancer immunity is poorly understood. Here, we use imaging-based deep learning to identify intratumoral cDC1-CD8⁺ T cell clustering as a unique feature of protective anti-cancer immunity. These clusters form selectively in stromal tumor regions and constitute niches in which cDC1 activate TCF1⁺ stem-like CD8⁺ T cells. We identify a distinct population of immunostimulatory CCR7^neg cDC1 that produce CXCL9 to promote cluster formation and cross-present tumor antigens within these niches, which is required for intratumoral CD8⁺ T cell differentiation and expansion and promotes cancer immune control. Similarly, in human cancers, CCR7^neg cDC1 interact with CD8⁺ T cells in clusters and are associated with patient survival. Our findings reveal an intratumoral phase of the anti-cancer T cell response orchestrated by tumor-residing cDC1 that determines protective versus ineffective immunity and could be exploited for cancer therapy.