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Stability and function of RCL1 are dependent on the interaction with BMS1
Yong Wang1, Zhenyu Zhao2, Hongyan Yu2
1Pathology Department of Taizhou Hospital, Zhejiang University, Taizhou 317000, China.
Journal of Molecular Cell Biology
|July 14, 2023
Summary
The BMS1/RCL1 complex is crucial for ribosome assembly and organ development. BMS1 protein stability and nucleolar entry of RCL1 depend on their interaction, essential for cell function.
Area of Science:
- Molecular Biology
- Cell Biology
- Genetics
Background:
- The small subunit (SSU) processome is vital for 40S ribosome assembly.
- The BMS1/RCL1 complex is a key SSU processome component involved in 18S rRNA processing.
- BMS1-like (Bms1l) and Rcl1 are essential for zebrafish digestive organ development.
Purpose of the Study:
- To investigate the mutual dependence of BMS1 and RCL1 for stability and function.
- To identify the molecular mechanisms underlying the BMS1-RCL1 interaction.
- To elucidate the role of BMS1-RCL1 interaction in ribosome biogenesis and organ development.
Main Methods:
- Identification of an RCL1-interacting domain in BMS1.
- Assessment of RCL1 protein stability and nucleolar entry in relation to BMS1.
- Functional rescue experiments in BMS1-knockdown cells and zebrafish mutants.
Main Results:
- An evolutionarily conserved RCL1-interacting domain was identified in BMS1.
- RCL1 stability and nucleolar localization are dependent on BMS1 interaction, preventing its degradation.
- Overexpression of RCL1 partially rescued 18S rRNA processing and cell proliferation defects in BMS1-knockdown cells.
- Hepatocyte-specific Rcl1 overexpression rescued liver development in a zebrafish bms1l substitution mutant, dependent on Rcl1's nucleolar entry.
Conclusions:
- BMS1-RCL1 interaction is essential for pre-ribosomal RNA processing and ribosome biogenesis.
- The complex regulates communication between ribosome biogenesis and cell cycle control.
- BMS1-RCL1 interaction is critical for maintaining protein stability, nucleolar localization, and overall cellular function.
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