Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

53
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
53
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

468
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
468

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Raman mapping on Ag-covered nanostructured substrates as an innovative approach for rapid estimation of post-mortem interval on human whole blood.

International journal of legal medicine·2026
Same author

Lactate-mediated NK cell dysfunction as a prognostic marker and therapeutic target in breast cancer.

Cell death discovery·2026
Same author

Molecular-level understanding of the aging bone and regeneration mechanisms using computational methods.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2026
Same author

Applications of Raman spectroscopy in the post-mortem interval estimation: a systematic literature review.

International journal of legal medicine·2026
Same author

A Fluid Dynamics-Model System for Advancing Tissue Engineering and Cancer Research Studies: Biological Assessment of the Innovative BioAxFlow Dynamic Culture Bioreactor.

Biomimetics (Basel, Switzerland)·2025
Same author

CCDC6 Immunostaining in Conjunction with the Rad51 HRD Assay May Expand PARPi Treatment Eligibility in Patients with HGSOC.

Cancer research communications·2025

Related Experiment Video

Updated: Jul 23, 2025

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing
11:02

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing

Published on: October 18, 2013

19.5K

Learning models for classifying Raman spectra of genomic DNA from tumor subtypes.

Giacomo Lancia1, Claudio Durastanti2, Cristian Spitoni1

  • 1Mathematical Institute, Utrecht University, Budapestlaan 6, 3584 CD, Utrecht, The Netherlands.

Scientific Reports
|July 14, 2023
PubMed
Summary

This study uses Surface Enhanced Raman Scattering (SERS) with silver-coated nanowires to detect different tumor DNA subtypes. This method aids in early cancer detection and personalized therapy guidance.

More Related Videos

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
07:37

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Published on: January 9, 2020

9.5K
Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
06:53

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

8.7K

Related Experiment Videos

Last Updated: Jul 23, 2025

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing
11:02

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing

Published on: October 18, 2013

19.5K
An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
07:37

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Published on: January 9, 2020

9.5K
Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
06:53

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

8.7K

Area of Science:

  • Biophysics
  • Nanotechnology
  • Genomics

Background:

  • Early tumor subtype detection is vital for personalized therapy and metastasis prediction.
  • Current methods may lack the specificity needed for precise subtyping.

Purpose of the Study:

  • To develop and validate a Surface Enhanced Raman Scattering (SERS) platform for discriminating between different tumor DNA subtypes.
  • To explore the potential of SERS in identifying distinct molecular interactions indicative of tumor origin.

Main Methods:

  • Utilized a Surface Enhanced Raman Scattering (SERS) platform featuring disordered silver-coated silicon nanowires (Ag/SiNWs).
  • Performed label-free Raman mapping of dried DNA solutions from melanoma and colon tumors on the Ag/NW mat.
  • Employed machine learning models for classification based on SERS spectral data.

Main Results:

  • Successfully discriminated between genomic DNA of different melanoma and colon tumor subtypes using the SERS platform.
  • Identified specific and distinct physico-chemical interactions between tumor DNA and the Ag/NW substrate.
  • Observed potential correlation between DNA methylation degree and SERS spectral signatures.

Conclusions:

  • The Ag/SiNWs SERS platform offers an efficient method for early and accurate detection of tumor DNA subtypes.
  • This approach holds promise for guiding personalized cancer therapy and predicting metastatic potential.
  • Further investigation into DNA methylation's role in SERS interactions is warranted.