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Related Experiment Video

Updated: Jul 23, 2025

Curtain Flow Column: Optimization of Efficiency and Sensitivity

Curtain Flow Column: Optimization of Efficiency and Sensitivity

Published on: June 12, 2016

Comparison of multi-column chromatography configurations through model-based optimization.

Aditya Pareek¹, Venkata Sudheendra Buddhiraju¹, Vishnu Swaroopji Masampally¹

¹TCS Research, Tata Research Development and Design Centre, Tata Consultancy Services, Pune, India.

Biotechnology Progress

|July 16, 2023

Summary

Model-based optimization shows that 3-column periodic counter-current chromatography (PCC) offers higher yield and productivity for monoclonal antibody (mAb) purification. However, 4-column PCC provides consistent performance under wash volume constraints.

Keywords:

continuous bioprocessing global optimization model-based optimization monoclonal antibody multi-column chromatography

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Area of Science:

Biopharmaceutical Manufacturing
Chemical Engineering
Process Optimization

Background:

Integrated continuous bioprocessing is the future of biopharmaceutical manufacturing.
Multi-column chromatography enhances productivity and capacity utilization for biomolecule capture.
Periodic counter-current chromatography (PCC) is a key technology for continuous processing.

Purpose of the Study:

To optimize two prevalent multi-column PCC designs (3-column and 4-column).
To evaluate performance based on monoclonal antibody (mAb) feed concentration and operational protocols.
To identify optimal configurations for yield, productivity, and capacity utilization.

Main Methods:

Model-based optimization using Radial basis function technique.
Analysis of 3-column and 4-column PCC configurations.
Varying mAb feed concentrations, cleaning-in-place (CIP), and equilibration durations.
Decision variables included superficial velocities and stage durations.

Main Results:

Without wash volume constraints, 3-column PCC outperformed 4-column PCC in productivity, yield, and capacity utilization.
At 1.2 mg/mL mAb feed, 3-column PCC achieved 0.024 mg/mL.s productivity, 0.94 yield, and 0.94 capacity utilization.
With wash volume constraints, 4-column PCC demonstrated consistent productivity and yield, albeit with lower capacity utilization.

Conclusions:

3-column PCC is superior for maximizing yield and productivity in mAb purification when wash volumes are not limited.
4-column PCC offers robustness and consistent performance under stricter operational constraints, making it suitable for specific manufacturing scenarios.