Determination of uric acid in serum by SERS system based on V_O-MnCo₂O₄/Ag nanozyme

The level of uric acid is crucial to human health. Octahedral oxygen vacancy MnCo₂O₄/Ag (V_O-MnCo₂O₄/Ag) nanozyme was successfully prepared by simple hydrothermal, calcination and self-reduction methods. V_O-MnCo₂O₄/Ag nanozyme is rich in Mn²⁺/Mn³⁺ and C_O²⁺/C_O³⁺ redox electron pairs, large specific surface area and oxygen vacancies. V_O-MnCo₂O₄/Ag nanozyme showed high uricase-like activity and peroxidase-like activity. At the same time, the SERS signal of the detected molecule could be significantly enhanced after the catalytic reaction of the V_O-MnCo₂O₄/Ag nanozyme. The K_m values of V_O-MnCo₂O₄/Ag nanozyme for H₂O₂ and TMB were 0.04 mM and 0.027 mM respectively. Based on the uric acid oxidase-like and peroxidase-like activities of V_O-MnCo₂O₄/Ag, we developed a label-free, sensitive, and reliable SERS uric acid detection system. The detection linear range of uric acid is 0.01 μM-1000 μM and the detection of limit is 7.8 × 10^-9 M. The results show that the sensing system has good accuracy, sensitivity, selectivity, and stability. It can be applied to the determination of samples under different conditions. This study provides profound insights into the design of enzyme-like activity regulation and SERS properties regulation of nanozymes, provides guidance for the study of reaction kinetics and catalytic mechanism of nanozymes, and has broad application prospects in the field of nanozymes and SERS sensing analysis.