Self-checking dual-modal aptasensor based on hybrid Z-scheme heterostructure of Zn-defective CdS/ZnS for...

Electrochemical detection methods have been widely used for trace target detection with satisfactory results. However, most of the existing electrochemical sensors rely only on single signal output, which inevitably suffer from the interference of the complex matrix of real samples. Herein, we proposed a dual-modal aptasensor for oxytetracycline assay with self-checking function by integrating photoelectrochemical (PEC) and electrochemical (EC) signal outputs in one analysis system. Zn-defective CdS/ZnS heterostructure was synthesized and served as the photo-electroactive substrate for constructing the biorecognition process, while methylene blue (MB) was used as a dual-functional probe to enhance both PEC and EC signals. Due to the high activity of Zn-defective CdS/ZnS heterojunction and the unique dual-modal signal readout strategy, the biosensing platform exhibits superior analytical performance with the relatively wide linear range (0.01-50 ng mL^-1), lower detection limits of 1.86 pg mL^-1 (PEC mode) and 3.08 pg mL^-1 (EC mode), as well as good selectivity, stability and reproducibility. The proposed dual-model analytical system with self-checking function is envisioned to provide a new approach for sensitive and reliable biosensing.