Development of Au₈ nanocluster-based fluorescent strip immunosensor for sensitive detection of aflatoxin B₁

Gold clusters with intriguing chemical/physical properties have great promise in applications such as sensing and bio-imaging due to their fascinating photoluminescence character. In this study, an immunofluorescence sensor based on levonorgestrel protected atomically precise Au₈ nanocluster (Au₈NC) for aflatoxin B₁ (AFB₁) detection was fabricated due to its strong carcinogenic and mutagenic effect on humans. The prepared polymer-Au₈NC nanospheres displayed bright luminescence and good stability in aqueous solution. The obtained AFB₁ fluorescent strip immunosensor achieved quantitative point-of-care detection of AFB₁ in less than 15 min, with high selectivity and detection limits down to 0.27 ng/mL. In addition, the recovery rates of AFB₁ from tea soup ranged from 96% to 105% with relative standard deviations less than 10%. This work not only realized high-sensitively fluorescent sensing for AFB₁, but also expanded the bio-applications of atomic-precise metal clusters.