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Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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Peptide Identification Using Tandem Mass Spectrometry01:33

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Mass Spectrometry: Overview01:19

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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
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Related Experiment Video

Updated: Jul 23, 2025

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
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Mass spectrometry-based techniques for single-cell analysis.

Xiangyi Xu1, Xuanxi Jiang1, Meiyun Shi1

  • 1School of Life and Pharmaceutical Sciences, Dalian University of Technology, Panjin, 124221, PR China. leiyin@dlut.edu.cn.

The Analyst
|July 17, 2023
PubMed
Summary
This summary is machine-generated.

Mass spectrometry enables highly sensitive and selective single-cell analysis of proteins and lipids. This review details four key mass spectrometry methods for understanding cellular heterogeneity in health and disease.

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Area of Science:

  • Biochemistry and Molecular Biology
  • Analytical Chemistry
  • Cell Biology

Background:

  • Cells are fundamental to organismal function, and their heterogeneity is crucial for understanding physiological and pathological processes.
  • Analyzing proteins and lipids at the single-cell and subcellular levels is vital but challenging due to small sample size and complex matrices.
  • High sensitivity and selectivity are essential for accurate single-cell analysis.

Purpose of the Study:

  • To review and discuss mass spectrometry-based methods for single-cell analysis.
  • To summarize recent advances in applying these techniques to cellular studies.
  • To provide a reference for researchers in the field of single-cell mass spectrometry.

Main Methods:

  • Electrospray ionization mass spectrometry (ESI-MS)
  • Laser desorption ionization mass spectrometry (LDI-MS)
  • Secondary ion mass spectrometry (SIMS)
  • Inductively coupled plasma mass spectrometry (ICP-MS)

Main Results:

  • Mass spectrometry offers high sensitivity, selectivity, and multiplexing capabilities for single-cell analysis.
  • The review details the application and recent advancements of ESI-MS, LDI-MS, SIMS, and ICP-MS in single-cell studies.
  • These techniques are powerful tools for qualitative and quantitative analysis of biomolecules within single cells.

Conclusions:

  • Mass spectrometry is a critical technology for advancing single-cell research.
  • The discussed methods provide valuable insights into cellular heterogeneity and function.
  • This review serves as a comprehensive resource for the application of mass spectrometry in single-cell analysis.