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Investigations in high-precision sorting.

J T Merrill, P N Dean, J W Gray

    The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
    |January 1, 1979
    PubMed
    Summary
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    This study improves cell sorting accuracy by selecting droplets with cells near the center. This method significantly reduces droplet position dispersion, enhancing precision in cell relocation for research applications.

    Area of Science:

    • Biotechnology
    • Cell Biology
    • Analytical Chemistry

    Background:

    • Cell sorting technologies are crucial for biological research and diagnostics.
    • Accurate droplet deflection is essential for precise cell relocation.
    • Random cell and particle presence in sorter jets causes trajectory dispersion.

    Purpose of the Study:

    • To investigate and improve the accuracy of droplet sorting for individual cell relocation.
    • To quantify the reduction in droplet position dispersion using a novel sorting strategy.

    Main Methods:

    • Utilized a modified Becton Dickinson Fluorescence-Activated Cell Sorter.
    • Implemented a method to select droplets with cells positioned centrally.
    • Tested the technique using Lewis lung tumor cells for sorting.

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    Main Results:

    • Reduced droplet position dispersion from over 3% to approximately 1% of the average deflection.
    • Achieved a typical deflection of 15 mm with a 50-micron nozzle diameter.
    • Demonstrated that sorting onto less-wettable surfaces further minimizes cell position uncertainty.

    Conclusions:

    • The developed cell sorting method significantly enhances positional accuracy.
    • Optimized droplet selection and surface properties are key to precise cell sorting.
    • This technique offers improved reliability for relocating individual cells in biological assays.