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Related Experiment Videos

Polymorphonuclear leukocytes, complement, and Trichophyton rubrum.

M V Dahl, R Carpenter

    The Journal of Investigative Dermatology
    |February 1, 1986
    PubMed
    Summary
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    Complement activation by Trichophyton rubrum generates chemotactic factors and enhances polymorphonuclear leukocyte (PMNL) interaction with fungi. Complement opsonization significantly boosts PMNL

    Area of Science:

    • Immunology
    • Mycology
    • Dermatophytosis

    Background:

    • Trichophyton rubrum is a common dermatophyte causing superficial fungal infections.
    • The role of the complement system and polymorphonuclear leukocytes (PMNL) in host defense against T. rubrum is not fully elucidated.
    • Understanding these interactions is crucial for developing effective therapeutic strategies.

    Purpose of the Study:

    • To investigate the role of complement activation in the host immune response to Trichophyton rubrum.
    • To assess the synergistic effects of complement and PMNL in controlling fungal growth and viability.

    Main Methods:

    • Incubation of T. rubrum with fresh human serum to activate complement.
    • Assessing chemotaxis of PMNL towards fungal components.

    Related Experiment Videos

  • Measuring fungal growth and metabolic activity (radiolabeled N-acetylglucosamine incorporation) after incubation with serum and PMNL.
  • Evaluating PMNL adherence and respiratory burst activity (chemiluminescence) in response to opsonized and unopsonized fungi.

    • Main Results:

    • Complement activation produced factors chemotactic for PMNL.
    • Complement opsonization significantly enhanced PMNL adherence to fungal hyphae and stimulated a faster respiratory burst.
    • Opsonized T. rubrum hyphae showed significantly inhibited metabolic activity when incubated with PMNL.
    • Opsonized fungal spores were killed by PMNL, preventing subsequent growth, unlike unopsonized spores or spores incubated with heat-inactivated serum.

    Conclusions:

    • Complement activation is essential for generating PMNL-attracting factors and facilitating PMNL-fungal interactions.
    • Complement-mediated opsonization significantly enhances the fungistatic and fungicidal capabilities of PMNL against T. rubrum.
    • The combined action of complement and PMNL represents a key host defense mechanism against dermatophyte infections.