Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell differentiation based on absorption and scattering.

W G Eisert

    The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
    |January 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    How to get from antiplatelet to antithrombotic treatment.

    American journal of therapeutics·2001
    Same author

    Near-field amplification of antithrombotic effects of dipyridamole through vessel wall cells.

    Neurology·2001
    Same author

    Noninvasive method for measuring thrombus formation in patients after peripheral angioplasty using three-dimensional B-mode and color-coded Doppler ultrasonography.

    Journal of clinical pharmacology·1997
    Same author

    The effects of heparin and annexin V on fibrin accretion after injury in the jugular veins of rabbits.

    Thrombosis and haemostasis·1993
    Same author

    Dipyridamole alone or combined with low-dose acetylsalicylic acid inhibits platelet aggregation in human whole blood ex vivo.

    British journal of clinical pharmacology·1990
    Same author

    Relationship between vessel wall 13-HODE synthesis and vessel wall thrombogenicity following injury: influence of salicylate and dipyridamole treatment.

    Thrombosis research·1990
    Same journal

    Expression and Spatial Localization of Glycosaminoglycan Types in Cartilage From Different Anatomical Locations.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
    Same journal

    Aquaporin-4 Localization in Terminal Glial Cells of Human Mechanosensory Corpuscles: An Immunohistochemical Study of Penile Tissues.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
    Same journal

    Seeing, Interpreting, and Trusting Images in the Age of Artificial Intelligence.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
    Same journal

    P63 and Tight Junctions Targeted for Therapy in Human Salivary Duct Adenocarcinoma Cells.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
    Same journal

    Human iPSC-Derived Blood Vessel Organoids for Studying Chronic Hypoxia-Induced Microvascular Dysfunction.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
    Same journal

    From Manual to Macro: A Reproducible Fiji Workflow for Semi-automated Collagen Fibril Diameter Quantification in Transmission Electron Microscopy.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
    See all related articles

    High-throughput flow cytometry enables precise cell length measurements down to 2 microns. This advanced technique effectively differentiates cell populations, including red blood cells, lymphocytes, and tumor cells, based on size and light scattering properties.

    Area of Science:

    • Biomedical Engineering
    • Cell Biology
    • Optical Physics

    Background:

    • Accurate and high-throughput cell analysis is crucial for biological research and diagnostics.
    • Traditional methods may lack the precision or speed required for detailed cell population differentiation.

    Purpose of the Study:

    • To enhance flow cytometry systems for precise, high-rate cell length measurements.
    • To utilize cell size and light scattering for differentiating various cell types and their responses.

    Main Methods:

    • Improvements to a flow system for calibrated cell length measurements (<2 microns) at high rates.
    • Utilizing optical index matching to maintain axial symmetry for forward scattered light.
    • Analyzing cell size, axial light loss, and scattering intensity at different angles.

    Related Experiment Videos

    Main Results:

    • Successful differentiation of red blood cells based on surface structures.
    • Distinguished lymphocyte populations from different regions of the rat lymphatic system.
    • Observed distinct data patterns in experimental tumor cells following chemical treatments.

    Conclusions:

    • The enhanced flow system provides powerful tools for cell population differentiation.
    • Cell size and light scattering parameters are effective for analyzing cell surface structures and treatment effects.
    • This technology advances high-throughput analysis of cellular characteristics.