Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

8.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.2K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

14.4K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
14.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Plasma trimethylamine N-oxide and related metabolites and overall survival of high-grade serous ovarian cancer: a nested case-control study.

European journal of nutrition·2026
Same author

2-Aminoadipic Acid mediates the association between BMI and Lipids: Evidence from the CLSA and BHS Cohorts and Mendelian randomization.

American journal of preventive cardiology·2026
Same author

nanoASM: Long-Read Allele-Specific DNA Methylation Profiling Enables Functional Annotation of Regulatory Noncoding Variants in Human Prostate Tissues.

bioRxiv : the preprint server for biology·2026
Same author

Alzheimer's disease proteome-wide association study implicates adaptive immunity and identifies risk genes LILRB1 and SIRPA.

Science translational medicine·2026
Same author

Comparative analysis of neuropsychological impairments between Cushing's disease and non-functioning pituitary adenoma patients: the roles of HPA axis hormones and inflammatory markers in a prospective cohort study.

Endokrynologia Polska·2026
Same author

In Situ Translation of Terminal Glycans into Covalent Readouts in Complex Biological Systems.

Analytical chemistry·2026

Related Experiment Video

Updated: Jul 23, 2025

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

14.5K

Enabling programmable dynamic DNA chemistry using small-molecule DNA binders.

Junpeng Xu1,2, Guan Alex Wang1, Lu Gao1

  • 1Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, Chengdu, Sichuan, 610064, P. R. China.

Nature Communications
|July 17, 2023
PubMed
Summary
This summary is machine-generated.

Small molecules binding to DNA can program dynamic DNA reactions. This binder-induced nucleic acid displacement (BIND) method accelerates the discovery and characterization of DNA-binding compounds.

More Related Videos

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.2K
Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

1.0K

Related Experiment Videos

Last Updated: Jul 23, 2025

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

14.5K
DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.2K
Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

1.0K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Chemical Biology

Background:

  • Small molecules interacting with DNA, via intercalation or minor groove binding, can modify DNA stability and function.
  • These interactions are foundational for developing therapeutic agents and biosensors.

Purpose of the Study:

  • To introduce and validate a novel method, binder-induced nucleic acid displacement (BIND), for programming dynamic DNA reactions.
  • To demonstrate BIND's utility in characterizing DNA binders and discovering new bioactive small molecules.

Main Methods:

  • Utilizing DNA strand displacement reactions quantitatively tuned by small-molecule DNA binders.
  • Developing a single, unbiased molecular platform for comprehensive characterization of DNA binders, assessing affinity and sequence selectivity.
  • Engineering a tandem BIND system for high-throughput screening of compound libraries.

Main Results:

  • Demonstrated quantitative control over DNA strand displacement reactions using small-molecule DNA binders.
  • Successfully characterized existing and identified novel DNA binders without specialized equipment.
  • Discovered 8 novel DNA binders from a library of 700 compounds using the tandem BIND assay.

Conclusions:

  • BIND offers an innovative approach to accelerate the discovery and characterization of bioactive small molecules targeting DNA.
  • The BIND platform provides an unbiased, equipment-free method for assessing critical DNA binder parameters.
  • BIND facilitates high-throughput screening for novel DNA-binding compounds, demonstrating its potential in drug discovery and chemical biology.