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Apoptosis pathways and osteoporosis: An approach to genomic analysis
Hsiao-Ling Huang1, Chung-Ken Wu2, Dai-Jia Wu3
1Department of Healthcare Management, Yuanpei University of Medical Technology, Hsinchu, Taiwan.
Background:
Osteoporosis is a disease of the bone system that causes a decrease in skeletal density and degrades skeletal tissue. Decreased bone quality, so that bones are easily broken, damaged and fractured, is an important public health problem. Previous studies have shown that the maintenance of adult bone mass is not only due to changes in bone marrow and bone cells. By regulating apoptosis, they change the lifespan of each individual. This study influences understanding of the function of apoptosis in the pathogenesis of osteoporosis and the importance of controlling the mechanisms of osteoporosis.
Methods:
On the National Institute of Biotechnology Information website, Gene Expression Omnibus (GEO) microarray data and GSE551495 GEO profiles were collected. The gene set enrichment analysis tool was used to confirm the enrichment of genetic sets in relation to the gene set. The collection of C2 gene sets is compiled from the KEGG (https://www.gsea-msigdb.org/gsea/msigdb/human/search.jsp and https://www.kegg.jp/kegg/) online database and REACTOME (https://www.gsea-msigdb.org/gsea/msigdb/human/search.jsp and https://reactome.org/) pathway analysis. The Search Tool for the Retrieval of Interaction Genes (STRING) website was used to construct and select proteins and genes. The comparative toxicological genomic database (CTD) tools can be used to predict the relationship between apoptosis, osteoporosis-related genes and interactions between central genes and osteoporosis.
Results:
These results generally expand our understanding of the path of apoptosis in osteoporosis. We have discovered genes CASP9, CASP8, CASP3, BAX and TP53 associated with osteoporosis. In activation of KEGG apoptosis and REACTOME, caspase activation through the extrinsic apoptotic signaling pathway is characterized by the identification of a subcollection of C2. Other STRINGs show the formation of protein networks and central gene selection, and CTD can accurately predict the relationship between these apoptosis pathways and central genes.
Conclusions:
Our research has highlighted the importance of the osteoporosis pathway associated with osteoporosis apoptosis with several analytical approaches. These results have broadened our understanding of the pathways of osteoporosis apoptosis. It is particularly possible to predict the sensitivity and vulnerability to osteoporosis.
Insights
This study reveals key apoptosis-related genes, including CASP9, CASP8, CASP3, BAX, and TP53, involved in osteoporosis pathogenesis. Understanding these pathways can help predict osteoporosis vulnerability.
Area of Science:
- Biomedical Science
- Genetics
- Molecular Biology
Background:
- Osteoporosis is a significant public health issue characterized by decreased bone density and degraded skeletal tissue, leading to fractures.
- The maintenance of adult bone mass involves complex cellular processes, including the regulation of apoptosis, which affects cell lifespan.
- Understanding apoptosis mechanisms is crucial for comprehending osteoporosis pathogenesis and developing effective control strategies.
Purpose of the Study:
- To investigate the role of apoptosis in the pathogenesis of osteoporosis.
- To identify specific genes and pathways associated with osteoporosis and apoptosis.
- To enhance the understanding of osteoporosis mechanisms for potential therapeutic targets.
Main Methods:
- Utilized Gene Expression Omnibus (GEO) microarray data (GSE551495) from the National Center for Biotechnology Information.
- Performed gene set enrichment analysis using KEGG and REACTOME databases for pathway analysis.
- Employed STRING and Comparative Toxicogenomics Database (CTD) tools to construct protein-gene networks and predict gene interactions.
Main Results:
- Identified key apoptosis-related genes associated with osteoporosis: CASP9, CASP8, CASP3, BAX, and TP53.
- Confirmed caspase activation via the extrinsic apoptotic signaling pathway through KEGG and REACTOME analyses.
- Established protein-gene networks and accurately predicted relationships between apoptosis pathways and central osteoporosis genes using STRING and CTD.
Conclusions:
- Highlighted the critical role of osteoporosis-associated apoptosis pathways through multi-analytical approaches.
- Broadened the understanding of osteoporosis apoptosis pathways, offering insights into disease mechanisms.
- Provided a foundation for predicting osteoporosis sensitivity and vulnerability based on identified pathways and genes.
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01:30Apoptosis
01:17The Extrinsic Apoptotic Pathway
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