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Vagococcus proximus sp. nov. and Vagococcus intermedius sp. nov., originating from modified atmosphere packaged

Per Johansson1, Elina Jääskeläinen1, Elina Säde1

  • 1Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Agnes Sjöbergin katu 2, 00014 Helsinki, Finland.

International Journal of Systematic and Evolutionary Microbiology
|July 18, 2023
PubMed

Insights

Two novel bacterial species, Vagococcus proximus and Vagococcus intermedius, were identified from broiler meat spoilage microbiomes. These new Vagococcus species were characterized using genomic and phenotypic analyses.

Area of Science:

  • Microbiology
  • Bacteriology
  • Food Science

Background:

  • Microbial spoilage of modified atmosphere packaged broiler meat poses food safety concerns.
  • Identification of unknown bacterial isolates is crucial for understanding foodborne microbiomes.

Purpose of the Study:

  • To taxonomically characterize three unknown bacterial isolates from broiler meat spoilage.
  • To propose novel species within the genus Vagococcus based on polyphasic analyses.

Main Methods:

  • Polyphasic taxonomy including genome-based analyses (ANI, dDDH, 16S rRNA sequencing).
  • Phenotypic characterization and chemotaxonomic profiling (cellular fatty acid analysis).
  • Genotyping for streptomycin resistance (rpsL gene analysis).

Main Results:

  • Two novel Vagococcus species, Vagococcus proximus sp. nov. (PNs007^T) and Vagococcus intermedius sp. nov. (STAA11^T), were identified.
  • Genomic analyses (ANI, dDDH) confirmed their novelty, with ANI values of 89.5% and 76.2-76.4% respectively.
  • Vagococcus proximus and Vagococcus intermedius exhibited distinct phenotypic traits, including ammonia production from arginine.

Conclusions:

  • The identified isolates represent two distinct, novel species within the Vagococcus genus.
  • These findings expand the known diversity of Vagococcus and contribute to understanding meat spoilage microbiomes.
  • The proposed species, Vagococcus proximus and Vagococcus intermedius, are formally named and characterized.
Keywords:
modified atmosphere packaged broiler meatMAPVagococcus intermedius sp. nov.Vagococcus proximus sp. nov

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