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Related Experiment Videos

Adherence, augmented adherence, and aggregation of polymorphonuclear leukocytes.

J J McGillen, J P Phair

    The Journal of Infectious Diseases
    |January 1, 1979
    PubMed
    Summary
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    Polymorphonuclear leukocyte adherence to nylon fibers does not require plasma activation. This process is distinct from plasma-dependent leukocyte aggregation and migration inhibition, which involve complement component C3.

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Polymorphonuclear leukocytes (PMNs) play a crucial role in the innate immune system.
    • Leukocyte adherence, aggregation, and migration are critical inflammatory processes.
    • These processes are often modulated by plasma factors, particularly complement activation.

    Purpose of the Study:

    • To investigate the plasma dependence of polymorphonuclear leukocyte adherence to nylon fibers.
    • To differentiate this adherence mechanism from other plasma-mediated leukocyte functions.
    • To explore the role of complement component C3 in plasma-activated leukocyte responses.

    Main Methods:

    • Incubation of polymorphonuclear leukocytes with nylon fibers.
    • Assessment of adherence in plasma-independent and plasma-dependent conditions.

    Related Experiment Videos

  • Comparison with complement-activating agents like zymosan.
  • Inhibition studies using antiserum to complement component C3 (anti-C3) and heat inactivation of plasma.

    • Main Results:

    • Polymorphonuclear leukocyte adherence to nylon fibers was found to be plasma-independent.
    • This adherence mechanism is distinct from augmented adherence, leukocyte aggregation, and inhibited random migration, which are plasma-dependent.
    • Nylon fibers did not activate plasma, unlike zymosan.
    • Anti-C3 serum inhibited zymosan-induced PMN aggregation, and heat inactivation blocked zymosan-induced adherence augmentation.

    Conclusions:

    • Leukocyte adherence to nylon fibers represents a unique, plasma-independent pathway.
    • This finding distinguishes it from complement-mediated inflammatory responses.
    • The complement system, specifically C3, is involved in plasma-activated leukocyte aggregation and adherence augmentation, but not in direct PMN adherence to nylon.