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Related Experiment Video

Updated: Jun 17, 2026

A Microfluidic Model of Biomimetically Breathing Pulmonary Acinar Airways

A Microfluidic Model of Biomimetically Breathing Pulmonary Acinar Airways

Published on: May 9, 2016

Biomimetic Human Lung Alveolar Interstitium Chip with Extended Longevity.

Kun Man¹, Jiafeng Liu¹, Cindy Liang¹

¹Department of Biomedical Engineering, University of North Texas, Denton, Texas 76207, United States.

ACS Applied Materials & Interfaces

|July 18, 2023

Summary

A new human lung alveolar interstitium chip mimics the lung's microenvironment, improving barrier function and enabling realistic toxicity testing for lung disease research.

Keywords:

alveolar interstitium lung chip matrix stiffness mechanical stretch nanofibrous membrane permeability

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Last Updated: Jun 17, 2026

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Published on: February 17, 2023

Author Spotlight: Developing a Microfluidic Lung-on-Chip Model for In-Depth Study of Human Immune Response and Infection Mechanisms

Author Spotlight: Developing a Microfluidic Lung-on-Chip Model for In-Depth Study of Human Immune Response and Infection Mechanisms

Published on: May 31, 2024

Area of Science:

Biomedical Engineering
Pulmonary Medicine
Toxicology

Background:

Current preclinical lung models lack the anatomical and physiological relevance of human alveoli.
This limits understanding of lung diseases and toxicity assessments.
There is a need for advanced models that replicate the human lung interstitium.

Purpose of the Study:

To develop a human lung alveolar interstitium chip that replicates key microenvironmental factors.
To enhance the physiological relevance of preclinical lung models.
To provide a platform for studying lung diseases and assessing toxicity.

Main Methods:

Developed a biomimetic chip with an electrospun nanofibrous membrane, mimicking the basement membrane.
Co-cultured epithelial cells with fibroblasts in 3D collagenous gels.
Incorporated interstitial fluid flow and breathing-like mechanical stretch.

Main Results:

The chip demonstrated significantly improved epithelial barrier function compared to transwell models.
A collagen I-fibrin blend matrix enhanced barrier function and model longevity beyond eight weeks.
Milled carbon nanotube toxicity assessments on the chip aligned with animal study findings.

Conclusions:

The developed human lung alveolar interstitium chip offers superior physiological relevance for preclinical lung research.
This model enhances epithelial barrier function and provides a robust platform for toxicity testing.
The chip represents a significant advancement for studying lung diseases and their mechanistic causes.