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Updated: Jul 23, 2025
11:14
Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders
Published on: April 14, 2015
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Effect of Atomic Layer Coating on the Stability of Solid Myoglobin Formulations
Caio H N Barros1, Manuel Alfaro1, Cormac Costello1
1National Institution for Bioprocessing Research and Training (NIBRT), Dublin A94 X099, Ireland.
Molecular Pharmaceutics
|July 19, 2023
Summary
Atomic layer (ALC) coatings significantly enhance the storage stability of solid protein formulations. These advanced coatings protect proteins like myoglobin, improving recovery and reducing degradation during storage.
Area of Science:
- Materials Science
- Biotechnology
- Pharmaceutical Sciences
Background:
- Solid protein formulations are susceptible to degradation during storage.
- Atomic layer (ALC) coating is a novel technique for material surface modification.
- Myoglobin serves as a model protein to assess stabilization strategies.
Purpose of the Study:
- To investigate the impact of ALC coatings on the physical properties and storage stability of solid protein powders.
- To evaluate the efficacy of aluminum oxide and silicon oxide ALC coatings.
- To compare the performance of ALC-coated proteins with uncoated controls.
Main Methods:
- Myoglobin-mannitol powders were prepared using lyophilization or spray drying.
- Samples were subjected to aluminum oxide or silicon oxide ALC coating.
- Analyzed physical properties (moisture, reconstitution time, structure, crystallinity, aggregation) before and after 3 months of storage under controlled conditions.
Main Results:
- ALC coatings improved the recovery of soluble native myoglobin by up to twofold compared to uncoated samples.
- Coated samples exhibited reduced protein aggregation and monomer loss after storage.
- Protein recovery was influenced by formulation, coating type, and drying method; moisture content correlated with monomer loss.
Conclusions:
- ALC coatings offer a promising strategy for stabilizing solid protein formulations during storage.
- This technology provides significant advantages over conventional uncoated protein powders.
- Further research can optimize ALC coating parameters for enhanced protein drug product stability.
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