Adding nanoparticles to improve emulsion efficiency and enhance microbial degradation in Pickering emulsions

Interfacial microbial degradation of alkane in Pickering emulsions stabilized by hydrophobic bacterial cells is a new mechanism for microbial degradation of water-insoluble chemicals, where both water-insoluble chemicals in the oil phase and water-soluble nutrients (such as nitrogen and phosphorus) in the water phase are bio-accessible to living microorganisms anchoring onto the oil-water interfaces. In the present work, super-hydrophobic Mycobacterium sp. (contact angle 168.6°) degradation of tetradecane was set up as a model. Addition of fumed SiO₂ particles (Aerosil® R974) as a new strategy was developed to enhance tetradecane degradation where the biodegradation rate (based on the accumulated biomass) increased by approximately 80%. The enhanced effect of SiO₂ particles on the tetradecane degradation attributed to the synergistic effect of SiO₂ particles on the emulsion efficiency of Pickering emulsions stabilized by bacterial cells and then on the enhancement of interfacial microbial degradation in Pickering emulsions. KEY POINTS: • Interfacial microbial degradation in bacterial cells stabilized Pickering emulsions. • Adding fumed SiO₂ particles to enhance microbial degradation of tetradecane. • Correlation relationship between emulsion efficiency and interfacial microbial degradation.