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Updated: Jul 23, 2025
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Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles
Published on: March 28, 2021
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Single Nanovesicles Tracking Reveals Their Heterogeneous Extracellular Adsorptions
Hua Sun1, Huibo Qi1, Wanting Hu1
1MOE Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Laboratory of Flexible Electronics Technology, Center for Synthetic and Systems Biology, Department of Chemistry, Tsinghua University, Beijing, 100084, China.
Small (Weinheim an Der Bergstrasse, Germany)
|July 19, 2023
Summary
This study reveals how nanovesicles (NVs) interact with cells by tracking their adsorption dynamics. Understanding these heterogeneous interactions is key for advancing nanomedicine therapeutics.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Cell Biology
Background:
- Nanomedicine utilizes nanovesicles (NVs), including liposomes and exosomes, for disease therapeutics.
- Analyzing the complex, dynamic interactions between NVs and cells, especially extracellular adsorption, is challenging.
- Current methods often lack the spatiotemporal resolution to capture transient NV-cell dynamics.
Purpose of the Study:
- To directly observe and characterize the heterogeneous extracellular adsorption of nanovesicles (NVs) onto cells.
- To investigate the influence of extracellular matrix components on NV-cell interactions.
- To provide insights into the spatiotemporal dynamics of NV-cell interactions for nanomedicine development.
Main Methods:
- Single nanovesicle (NV) tracking on HCT116 cells.
- Classification of NV adsorption based on diffusion properties and cellular location.
- Enzymatic hydrolysis of extracellular hyaluronic acid to assess its impact on NV adsorption.
Main Results:
- NVs exhibit heterogeneous adsorption patterns on cells, with stable adsorption on the rear and restricted adsorption on the front.
- Hydrolysis of extracellular hyaluronic acid significantly weakens restricted NV adsorption, leading to dissociative adsorption.
- Single-cell, single-particle analysis revealed reduced overall NV adsorption after hyaluronic acid hydrolysis.
Conclusions:
- Direct spatiotemporal tracking reveals heterogeneous nanovesicle-cell adsorption dynamics.
- Extracellular matrix components modulate nanovesicle adsorption, impacting therapeutic delivery.
- These findings offer valuable insights for designing advanced nanomedicine and nano-investigations.
Keywords:
cellular protrusionextracellular adsorptionsextracellular matrixnanovesiclessingle particle tracking
