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Updated: Jul 23, 2025

Evaluation of Integrated Anaerobic Digestion and Hydrothermal Carbonization for Bioenergy Production
Published on: June 15, 2014
Revealing the roles of carbonized humic acid in biohydrogen production
Kexin Tian1, Jishi Zhang1, Chen Zhou1
1College of Environmental Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China.
Abstract:
For low yield in dark fermentation (DF), in this study, the carbonized humic acid (CHA) was produced and added to DF for enhancing biohydrogen (bioH2) yield at mesophilic condition. The highest bioH2 yield was 151.08 mL/g glucose with the addition of CHA at 80 mg/L, which was 35.27% and 16.53% higher than those of 0 mg/L CHA and 80 mg/L mineral humic acid (MHA) groups, respectively. Electrons preferentially conducted via the butyrate pathway due to CHA amendments, which corresponded to the prediction of relevant functional genes. Furthermore, CHA possessed distinctive advantages over MHA, which acted as an electron shuttle to facilitate electron transfer, released metal ions as an essential signal mediator and favored the reduction of ferredoxin, obtaining more H2. The use of CHA in the field of H2-DF depicted the high-value utilization and industrial chain extension of MHA.
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