Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Membrane electrical parameters in turtle bladder measured using impedance-analysis techniques.

C Clausen, T E Dixon

    The Journal of Membrane Biology
    |January 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Child and adolescent psychiatry training and mental health care in Southeast Europe.

    European child & adolescent psychiatry·2019
    Same author

    SU-E-T-598: Clinical Experience of Configuration, Commission and Implementation for SmartArc with MOSAIQ R&V System.

    Medical physics·2017
    Same author

    Do referrals work? Responses of childbearing newcomers to referrals for care.

    Journal of immigrant and minority health·2009
    Same author

    Quad-Q-learning.

    IEEE transactions on neural networks·2008
    Same author

    Multiple ovulation and embryo transfer with fresh, frozen and vitrified red deer (Cervus elaphus) embryos in Argentina.

    Animal reproduction science·2007
    Same author

    Interspecific embryo transfer from mouflon (Ovis gmelini musimon) to domestic Corriedale sheep in Argentina.

    Animal reproduction science·2007
    Same journal

    In vitro Reconstitution of Syncytin-2 and MFSD2A Reveals their Functional Tethering in Membrane Fusion Initiation.

    The Journal of membrane biology·2026
    Same journal

    Structural Basis of SERCA Inhibition by Derivatives of di-tert-butylhydroquinone Revealed by X-ray Crystallography.

    The Journal of membrane biology·2026
    Same journal

    Fungal Extracellular Vesicles are Recoverable Across Variable Ultracentrifugation Speeds but Display Species-specific Profiles of Sedimentation.

    The Journal of membrane biology·2026
    Same journal

    Polyproline Modulates Membrane Translocation of Arginine-Rich Cell-Penetrating Peptides: Insights from Molecular Dynamics Simulations.

    The Journal of membrane biology·2026
    Same journal

    Peculiarities of Phosphatidylserine Externalization by Nano- and Microsecond Electric Pulses.

    The Journal of membrane biology·2026
    Same journal

    Protonation of Key Acidic Residues Reveals Binding Features of PCABs to Gastric H, K-ATPase.

    The Journal of membrane biology·2026
    See all related articles

    Equivalent-circuit impedance analysis reveals how membrane properties in turtle bladders change with proton secretion. This method quantifies membrane area and conductance changes linked to transport regulation.

    Area of Science:

    • Physiology
    • Membrane Biophysics
    • Renal Physiology

    Background:

    • Urinary bladders regulate ion and fluid balance.
    • Proton secretion is a key function in some species.
    • Understanding membrane dynamics is crucial for transport regulation.

    Purpose of the Study:

    • To quantify membrane ionic conductances and areas in turtle bladders.
    • To investigate the association between membrane parameters and proton secretion rate.
    • To validate a method for measuring dynamic membrane area changes.

    Main Methods:

    • Equivalent-circuit impedance analysis of turtle urinary bladders.
    • Inhibition of sodium reabsorption using amiloride and ouabain.
    • Manipulation of bathing solutions (CO2, HCO3-) and serosal oncotic pressure (albumin).

    Related Experiment Videos

    Main Results:

    • A simple equivalent-circuit model accurately represents transepithelial impedance.
    • Inhibition of sodium transport caused a time-dependent decline in membrane conductances and capacitances, likely due to cell volume changes.
    • Stable membrane parameters were achieved without ouabain and with increased serosal oncotic pressure.
    • Acetazolamide decreased apical membrane area, while CO2/HCO3- increased both apical membrane area and specific conductance.

    Conclusions:

    • Equivalent-circuit impedance analysis effectively quantifies membrane parameters in turtle bladders.
    • Changes in apical membrane area are directly related to the rate of proton transport.
    • This method allows quantification of membrane area changes due to endocytosis/exocytosis, impacting proton transport regulation.