Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

828
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
828
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

5.0K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
5.0K
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

5.4K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
5.4K
Mass Spectrometers01:16

Mass Spectrometers

5.7K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
5.7K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.6K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.6K
Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

655
The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
655

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epigenetic and oncogenic inhibitors converge to drive a metabolic catastrophe in castration-resistant prostate cancer.

The Journal of clinical investigation·2026
Same author

Volume of distribution in pharmacokinetics and clinical medicine: a critical update.

The Journal of pharmacy and pharmacology·2026
Same author

MIAAIM: Multi-omics image integration with dimensional reduction for tissue state mapping.

PLoS computational biology·2026
Same author

Author Correction: In vitro characterization of the human segmentation clock.

Nature·2026
Same author

Aging dictates tumor-specific genomic alterations across cancer types.

npj aging·2026
Same author

iPSC-derived skeletal muscle spheroids for Duchenne Muscular Dystrophy modeling.

Skeletal muscle·2026
Same journal

Biodegradable Self-Powered Electrotherapy Patch for Integrated Smart Wound Management.

Analytical chemistry·2026
Same journal

Metabolite Fraction Libraries for Quantitative NMR Metabolomics.

Analytical chemistry·2026
Same journal

Self-Contained Lateral-Flow Microfluidic Bead-Based Assay for Rapid Quantification of Early-Stage Kidney Biomarkers.

Analytical chemistry·2026
Same journal

Overcoming the Debye Shielding Effect with Concave-Convex Structures for Sensitivity-Enhanced Thin-Film Transistors.

Analytical chemistry·2026
Same journal

Mode-Phase-Difference Photothermal Spectroscopy Assisted by a Bent Biconically Tapered Microfiber for Gas Sensing.

Analytical chemistry·2026
Same journal

Negative-Pressure-Actuated Microfluidics: A Dual-Mode Point-of-Care Sensor for Allergen-Specific IgE in Interstitial Fluid.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Jul 23, 2025

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

21.0K

Chemical QuantArray: A Quantitative Tool for Mass Spectrometry Imaging.

Sylwia A Stopka1,2, Daniela Ruiz1,3, Gerard Baquer1

  • 1Department of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, United States.

Analytical Chemistry
|July 20, 2023
PubMed
Summary
This summary is machine-generated.

Chemical QuantArray enhances matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) for metabolite quantification. This novel method improves precision and accuracy for clinical applications by creating calibration curves directly on tissue microarrays.

More Related Videos

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

6.9K
Quantitative Analysis of Chromatin Proteomes in Disease
08:11

Quantitative Analysis of Chromatin Proteomes in Disease

Published on: December 28, 2012

13.2K

Related Experiment Videos

Last Updated: Jul 23, 2025

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

21.0K
Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

6.9K
Quantitative Analysis of Chromatin Proteomes in Disease
08:11

Quantitative Analysis of Chromatin Proteomes in Disease

Published on: December 28, 2012

13.2K

Area of Science:

  • Analytical Chemistry
  • Biomedical Imaging
  • Metabolomics

Background:

  • Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) offers spatial metabolite detection but needs better clinical translation.
  • Current MALDI-MSI methods face challenges in throughput, precision, and accuracy for clinical applications.

Purpose of the Study:

  • To develop an improved method for precise and accurate metabolite quantification using MALDI-MSI.
  • To enhance the clinical feasibility of MALDI-MSI for analyzing tissue specimens.

Main Methods:

  • Developed "Chemical QuantArray", a gelatin tissue microarray (TMA) mold with serial dilutions of isotopically labeled metabolite standards.
  • Cryo-sectioned TMAs onto tissue homogenate to generate calibration curves.
  • Implemented automated pixel removal and investigated intensity normalization strategies, including internal standards.

Main Results:

  • Chemical QuantArray enables wide dynamic range quantification of endogenous metabolites.
  • The technique reduces calibration standard space on MALDI slides by ~80%.
  • Achieved precision (<20% RSD) and accuracy (<20% DEV) through pixel removal and normalization.
  • Successfully quantified multiple purine metabolites in 14 clinical tumor specimens on a single slide.

Conclusions:

  • Chemical QuantArray significantly improves the analytical performance of MALDI-MSI metabolite quantification.
  • This method enhances the practical feasibility for clinical and translational research.
  • The technique offers a more efficient and reliable approach for spatial metabolomics in complex biological samples.