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Updated: Jul 23, 2025

Fabrication of Thin Film Silver/Silver Chloride Electrodes with Finely Controlled Single Layer Silver Chloride
Published on: July 1, 2020
Screen-Printed Silver/Silver Chloride Electrodes Inhibit Alcohol Oxidase Activity
Bob M Lansdorp1, Peter Lamberg1, Rashad Hamid1
1Milo Sensors, Inc. Santa Barbara, CA, 93101, United States of America.
Abstract:
Silver/silver chloride (Ag/AgCl) is ubiquitous in the field of electrochemical biosensing due to its suitability as a reference electrode material. However, we recently discovered that screen-printed Ag/AgCl ink has a detrimental effect on Alcohol Oxidase enzyme stability. We performed an optical absorbance assay to isolate the interaction of enzyme and electrode to discover a surprisingly strong inhibition effect. The halftime of enzymatic activity was reduced from nearly 1 week in buffer to 10 h in the presence of the Ag/AgCl electrode. We expect this discovery to have broad implications on enzymatic biosensors that use Ag/AgCl as reference electrode material.
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