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microRNA-651-5p affects the proliferation, migration, and invasion of lung cancer cells by regulating Calmodulin 2

Yaoguo Lang1, Xianglong Kong1, Benkun Liu1

  • 1Department of Thoracic Surgery, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, China.

The Clinical Respiratory Journal
|July 20, 2023
PubMed
Abstract

Insights

MicroRNA-651-5p (miR-651-5p) is downregulated in lung cancer and inhibits tumor growth. It targets Calmodulin 2 (CALM2), suppressing lung cancer cell proliferation, migration, and invasion.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Lung cancer is a major global health concern, responsible for significant mortality.
  • Understanding the molecular mechanisms driving lung cancer progression is crucial for developing effective therapies.

Purpose of the Study:

  • To investigate the role of the microRNA-651-5p (miR-651-5p) and Calmodulin 2 (CALM2) interaction in lung cancer.
  • To elucidate the molecular mechanism by which miR-651-5p/CALM2 axis influences lung cancer cell proliferation, migration, and invasion.

Main Methods:

  • Comparative analysis of miR-651-5p and CALM2 expression in lung cancer versus adjacent normal tissues.
  • Correlation analysis between miR-651-5p expression and clinicopathological features of lung cancer patients.
  • In vitro assays to assess the impact of miR-651-5p and CALM2 on lung cancer cell behavior.

Main Results:

  • miR-651-5p was found to be significantly downregulated in lung cancer tissues and cells.
  • Lower miR-651-5p expression correlated with advanced tumor size, TNM stage, and lymph node metastasis.
  • Overexpression of miR-651-5p suppressed lung cancer cell proliferation, migration, and invasion.
  • miR-651-5p directly targets CALM2, and CALM2 overexpression can reverse the inhibitory effects of miR-651-5p.

Conclusions:

  • The miR-651-5p/CALM2 axis plays a critical role in regulating the malignant behaviors of lung cancer cells.
  • miR-651-5p acts as a tumor suppressor by downregulating CALM2, thereby inhibiting lung cancer progression.
Keywords:
CALM2apoptosisinvasionlung cancermicroRNA-651-5pmigrationproliferation

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