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Updated: Jul 22, 2025
CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
Published on: April 25, 2022
Circ_0000419 acts as a tumor suppressor in gastric cancer development via regulating miR-300/RGMB axis
Jiajun Lu1, Yuan Zhou1, Zhiheng Chen1
1Department of Gastrointestinal Surgery, The First Hospital of Jiaxing, The Affiliated Hospital of Jiaxing University, No. 1882 Zhonghuan South Road, Jiaxing, Zhejiang, China.
Objective:
Dysregulated circular RNAs (circRNAs) have been verified to function in the development of gastric cancer (GC). The current study was designed to investigate the role of circ_0000419 in GC progression, and the potential mechanistic pathway.
Methods:
Relative expression of circ_0000419, microRNA-300 (miR-300) and Repulsive Guidance Molecule B (RGMB) was analyzed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assay. Cell metastasis, including migration and invasion, was assessed by wound healing and Transwell assays. Glucose consumption and lactate production were examined using kits. The association between miR-300 and circ_0000419 or RGMB was validated by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assays. Role of circ_0000419 in vivo was determined by xenograft experiment.
Results:
Circ_0000419 and RGMB were downregulated, while miR-300 was upregulated in GC tissues and cells. Gain of circ_0000419 inhibited migration, invasion and glycolysis in GC cells, which was attenuated by introduction of miR-300 or silencing of RGMB. Circ_0000419 sponged miR-300, and RGMB was direct target of miR-300. Circ_0000419 overexpression could block GC tumor growth in vivo.
Conclusion:
Circ_0000419 inhibited GC cell migration, invasion and glycolysis through regulation of miR-300/RGMB axis, at least in part, affording a molecular target for GC treatment.
Insights
Circular RNA circ_0000419 inhibits gastric cancer progression by regulating the miR-300/RGMB pathway. This finding offers a potential molecular target for gastric cancer treatment.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Circular RNAs (circRNAs) are increasingly recognized for their roles in cancer development.
- Dysregulation of circRNAs is implicated in the pathogenesis of gastric cancer (GC).
Purpose of the Study:
- To investigate the role of circ_0000419 in gastric cancer progression.
- To elucidate the underlying mechanistic pathway involving circ_0000419.
Main Methods:
- Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for gene expression analysis.
- Cellular assays (wound healing, Transwell) for metastasis assessment.
- Biochemical assays for glycolysis.
- Dual-luciferase reporter and RNA immunoprecipitation (RIP) assays for molecular interactions.
- In vivo xenograft experiments for tumor growth evaluation.
Main Results:
- Circ_0000419 and RGMB were downregulated, while miR-300 was upregulated in GC tissues and cells.
- Overexpression of circ_0000419 suppressed GC cell migration, invasion, and glycolysis.
- These inhibitory effects were reversed by miR-300 upregulation or RGMB silencing.
- Circ_0000419 acts as a sponge for miR-300, and RGMB is a direct target of miR-300.
- In vivo, circ_0000419 overexpression inhibited tumor growth.
Conclusions:
- Circ_0000419 inhibits gastric cancer cell migration, invasion, and glycolysis.
- This inhibition occurs via the regulation of the miR-300/RGMB axis.
- Circ_0000419 represents a potential molecular target for gastric cancer therapy.
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