[Limits of detection of nuclear DNA aneuploidy by FCM]

The limits of FCM detection of nuclear DNA aneuploidy were determined by simulation of the relationship between the coefficient of variation (CV) and DNA difference of two populations using a microcomputer. Under ideal conditions, the minimal DNA difference necessary for a bimodal peak is almost twice the CV. When the DNA difference is below the value, a single peak with a large CV and/or a shoulder is formed. Cases with a larger CV than expected in a single population should be practically treated as a population mixed with the near-diploid line.