Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Salmonella Typhi gut invasion drives hypoxic immune subsets associated with disease outcomes.

Nature communications·2025
Same author

Clonal hematopoiesis-associated motoric deficits caused by monocyte-derived microglia accumulating in aging mice.

Cell reports·2025
Same author

Early and delayed STAT1-dependent responses drive local trained immunity of macrophages in the spleen.

eLife·2025
Same author

Boycotting academics in Israel is counterproductive.

Nature·2024
Same author

Protocol for comparing ribosomal levels in single bacterial cells at different growth stages using rRNA-FISH.

STAR protocols·2024
Same author

Single-Molecule Fluorescent In Situ Hybridization (smFISH) for RNA Detection in Bacteria.

Methods in molecular biology (Clifton, N.J.)·2024

Related Experiment Video

Updated: Jul 22, 2025

Analyzing Ex Vivo Metabolic Flux in Splenic and Cardiac Macrophages and Bone Marrow Monocytes
06:26

Analyzing Ex Vivo Metabolic Flux in Splenic and Cardiac Macrophages and Bone Marrow Monocytes

Published on: March 28, 2025

346

Tissue-specific macrophage immunometabolism.

Hadar Ben-Arosh1, Roi Avraham1

  • 1Department of Immunology and Regenerative Biology, Weizmann Institute of Science, Rehovot 7610001, Israel.

Current Opinion in Immunology
|July 20, 2023
PubMed
Summary
This summary is machine-generated.

Gut macrophages dynamically adjust their metabolism to maintain tissue balance. Understanding these metabolic shifts, influenced by microbiota and tissue signals, is key for developing targeted therapies for gut inflammation and infection.

More Related Videos

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation
07:46

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Published on: April 3, 2017

25.3K
Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis
07:45

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Published on: November 28, 2015

35.6K

Related Experiment Videos

Last Updated: Jul 22, 2025

Analyzing Ex Vivo Metabolic Flux in Splenic and Cardiac Macrophages and Bone Marrow Monocytes
06:26

Analyzing Ex Vivo Metabolic Flux in Splenic and Cardiac Macrophages and Bone Marrow Monocytes

Published on: March 28, 2025

346
Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation
07:46

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Published on: April 3, 2017

25.3K
Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis
07:45

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Published on: November 28, 2015

35.6K

Area of Science:

  • Immunology
  • Cellular Metabolism
  • Gastroenterology

Background:

  • Macrophages are crucial immune cells that maintain tissue homeostasis by sensing their environment.
  • Immune cell function, particularly macrophage activity, is tightly regulated by energy metabolism (immunometabolism).
  • While cell-intrinsic factors like succinate and itaconate are known immunomodulators, cell-extrinsic signals also shape macrophage metabolism in a tissue-specific manner.

Purpose of the Study:

  • This review focuses on macrophage immunometabolism within the gut, a metabolically and immunologically complex environment.
  • It aims to explore how gut macrophages adapt their metabolism in response to microbial and host-derived signals during homeostasis and disease.
  • The review highlights the importance of spatial analysis for understanding macrophage metabolic niches in the gut.

Main Methods:

  • The review synthesizes existing literature on macrophage immunometabolism in the gut.
  • It discusses findings related to the influence of microbiota-derived metabolites and pro-inflammatory ligands.
  • Emphasis is placed on the utility of spatial analysis techniques for characterizing macrophage metabolic states.

Main Results:

  • Gut macrophages exhibit significant metabolic plasticity, adapting to the unique microenvironment.
  • Microbiota-derived metabolites play a critical role in balancing inflammatory signals, influencing macrophage metabolic programming.
  • Distinct metabolic niches for macrophage subsets exist within the gut tissue.

Conclusions:

  • Understanding the metabolic adaptations of gut macrophages is essential for comprehending gut immunity.
  • Identifying metabolic perturbations in specific macrophage subsets during inflammation or infection can guide the development of novel metabolotherapies.
  • Tissue-specific approaches are crucial for targeting macrophage metabolism effectively in the gut.