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Generation of control iPSC lines CBRCULi008-A and CBRCULi009-A derived from lymphoblastoid cell lines

Charles-Albert Chapotte-Baldacci1, Dominic Jauvin1, Mohamed Chahine1

  • 1CERVO Brain Research Centre, Institut Universitaire en Santé Mentale de Québec, Quebec City, QC G1J 2G3, Canada; Department of Medicine, Université Laval, Quebec City, QC G1V 0A6, Canada.

Stem Cell Research
|July 20, 2023
PubMed

Insights

We generated two control human induced pluripotent stem cell (hiPSC) lines for fundamental research. These hiPSCs are well-characterized and suitable for studying human disease mechanisms.

Area of Science:

  • Stem cell biology
  • Human iPSC generation

Background:

  • Control human induced pluripotent stem cell (hiPSC) lines are crucial for understanding human disease mechanisms.
  • Characterized hiPSC lines are needed for robust physiological and physiopathological studies.

Purpose of the Study:

  • To generate and characterize novel control human induced pluripotent stem cell (hiPSC) lines.
  • To provide essential tools for fundamental research into human diseases.

Main Methods:

  • Generation of hiPSC lines from lymphoblastoid cells of healthy donors.
  • Comprehensive characterization including pluripotency marker analysis, differentiation potential, karyotyping, and assessment of reprogramming vectors.

Main Results:

  • Successfully generated two distinct control hiPSC lines.
  • Confirmed pluripotency marker expression and differentiation capacity into three embryonic germ layers.
  • Verified normal karyotypes and colony morphology, with no residual reprogramming viral vectors.

Conclusions:

  • The generated hiPSC lines are well-characterized and possess key attributes for pluripotency.
  • These control hiPSC lines serve as valuable resources for fundamental research in human physiology and disease.

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