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Comparative transcriptome analysis reveals the key genes and pathways involved in drought stress response of two
Yufei Niu1, Jingyu Li1, Fanting Sun1
1College of Agronomy, Liaocheng University, Liaocheng 252059, PR China.
Abstract:
Drought stress results in significant yield losses in wheat production. Although studies have reported a number of wheat drought tolerance genes, a deeper understanding of the tolerance mechanisms is required for improving wheat tolerance against drought stress. In this study, we found that "Deguo 2" exhibited higher tolerance to drought than "Truman". Transcriptomics analysis enabled identification of 6084 and 7146 differentially expressed genes (DEGs), mainly mapping flavonoid biosynthesis, plant hormone, phenolamides and antioxidant pathways and revealed altered expression levels of about 700 genes. Exogenous melatonin application enhanced wheat tolerance against drought stress. Co-expression analysis showed that bHLH and bZIP transcription factors may be involved in the regulation of various pathway genes. Take together, these results provide new insights for us on exploring the crosstalk between phytohormones and secondary metabolites, and will deepen the understanding of the complex tolerance mechanisms against drought stress in wheat.
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Transcription
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
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