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A comparison of two methods for quantifying D-glucaric acid.

K K Steinberg, L L Needham

Journal of Analytical Toxicology

|July 1, 1986

Summary

The enzyme inhibition method is preferred for quantifying urinary D-glucaric acid over the glyoxylic acid method. It is more reliable due to less interference and is less labor-intensive.

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Area of Science:

Biochemistry
Analytical Chemistry
Clinical Chemistry

Background:

Urinary D-glucaric acid is a biomarker for certain metabolic processes.
Accurate quantification of D-glucaric acid is essential for clinical and research applications.
Existing spectrophotometric methods have limitations in accuracy and labor intensity.

Purpose of the Study:

To evaluate and compare the relative usefulness of two spectrophotometric methods for quantifying urinary D-glucaric acid.
To identify the most reliable and efficient method for routine analysis.

Main Methods:

Enzyme inhibition method: Measures beta-D-glucuronidase inhibition by 1,4-glucarolactone derived from urinary D-glucaric acid.
Glyoxylic acid method: Involves ion exchange chromatography separation followed by oxidation of D-glucaric acid to glyoxylic acid.
Both methods were applied to urine samples from 61 healthy adults and standard solutions.

Main Results:

Both methods yielded similar results for standard solutions with approximately 105% recovery.
Urinary D-glucaric acid concentrations were 3-10 times higher with the glyoxylic acid method compared to the enzyme inhibition method.
Higher values in the glyoxylic acid method were attributed to interference from ascorbic acid and other compounds.

Conclusions:

The modified enzyme inhibition method is preferable for quantifying urinary D-glucaric acid.
The enzyme inhibition method demonstrates greater specificity and is less labor-intensive than the glyoxylic acid method.
Interference from ascorbic acid significantly impacts the accuracy of the glyoxylic acid method.

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