Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Multi-layered dosage compensation of the avian Z chromosome by increased transcriptional burst frequency and elevated translational rates.

Nature communications·2025

Same author

Introducing synthetic thermostable RNase inhibitors to single-cell RNA-seq.

Nature communications·2024

Same author

All-optical voltage imaging-guided postsynaptic single-cell transcriptome profiling with Voltage-Seq.

Nature protocols·2024

Same author

Cholinergic Control of GnRH Neuron Physiology and Luteinizing Hormone Secretion in Male Mice: Involvement of ACh/GABA Cotransmission.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2024

Same author

Soluble and multivalent Jag1 DNA origami nanopatterns activate Notch without pulling force.

Nature communications·2024

Same author

Multivalent insulin receptor activation using insulin-DNA origami nanostructures.

Nature nanotechnology·2023

Same journal

RNAbpFlow: base pair-augmented SE(3) flow matching for conditional RNA 3D structure generation.

Nature methods·2026

Same journal

Spatio-DARLIN enables robust and efficient in situ lineage tracing in mice at single-cell resolution.

Nature methods·2026

Same journal

EasyGrid: a versatile platform for automated cryo-EM sample preparation and quality control.

Nature methods·2026

Same journal

Cloud-based microscope enables live neuroimaging for 24 h and beyond with worldwide access.

Nature methods·2026

Same journal

Deep molecular profiling in three dimensions.

Nature methods·2026

Same journal

3D pathology-guided microdissection.

Nature methods·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 5, 2026

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

Published on: May 25, 2011

Voltage-Seq: all-optical postsynaptic connectome-guided single-cell transcriptomics.

Veronika Csillag¹, Marianne Hiriart Bizzozzero¹, J C Noble²

¹Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.

|July 20, 2023

Summary

This summary is machine-generated.

Voltage-Seq enables targeted neuron selection for single-cell RNA sequencing by classifying postsynaptic response types (PRTs). This method links neuronal connections to gene expression, advancing connectome research.

More Related Videos

Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing DIVA-Seq

Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing DIVA-Seq

Published on: October 26, 2018

Ex Vivo Optogenetic Interrogation of Long-Range Synaptic Transmission and Plasticity from Medial Prefrontal Cortex to Lateral Entorhinal Cortex

Ex Vivo Optogenetic Interrogation of Long-Range Synaptic Transmission and Plasticity from Medial Prefrontal Cortex to Lateral Entorhinal Cortex

Published on: February 25, 2022

Related Experiment Videos

Last Updated: May 5, 2026

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

Published on: May 25, 2011

Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing DIVA-Seq

Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing DIVA-Seq

Published on: October 26, 2018

Ex Vivo Optogenetic Interrogation of Long-Range Synaptic Transmission and Plasticity from Medial Prefrontal Cortex to Lateral Entorhinal Cortex

Ex Vivo Optogenetic Interrogation of Long-Range Synaptic Transmission and Plasticity from Medial Prefrontal Cortex to Lateral Entorhinal Cortex

Published on: February 25, 2022

Area of Science:

Neuroscience
Molecular Biology
Systems Neuroscience

Background:

Characterizing neuronal connections is crucial for understanding information flow.
Postsynaptic response types (PRTs) are diverse but difficult to link with gene expression due to low-throughput electrophysiology and lack of scRNA-seq selection criteria.

Purpose of the Study:

To develop a method for targeting neurons based on specific PRTs for subsequent single-cell RNA sequencing (scRNA-seq).
To overcome the limitations of traditional electrophysiology and integrate functional response with genetic profiling.

Main Methods:

Developed Voltage-Seq, an all-optical voltage imaging technique.
Recorded PRTs of 8,347 neurons in the mouse periaqueductal gray (PAG) evoked by optogenetic activation of ventromedial hypothalamic (VMH) terminals.
Created VoltView, an onsite analysis tool with a classifier for navigating soma harvesting towards target PRTs using the VMH-PAG connectome database.

Main Results:

Classified and spatially resolved PRTs within the VMH-PAG connectome.
Successfully demonstrated Voltage-Seq by identifying VMH-driven γ-aminobutyric acid-ergic neurons in the PAG based solely on onsite VoltView classification.
Established a link between functional neuronal activity (PRTs) and genetic identity (scRNA-seq).

Conclusions:

Voltage-Seq provides a high-throughput method to functionally characterize neuronal populations.
This technique enables targeted cell harvesting for scRNA-seq, bridging the gap between neuronal connectivity, function, and gene expression.
Voltage-Seq significantly advances the study of neural circuits and connectomics.