Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.4K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Refractive-index contrast boosts scattering in gel wax phantoms: multi-material validation of a Δ<i>n</i> > 0.5 threshold.

Applied optics·2026
Same author

Automated FerryBox monitoring reveals the first recorded river induced crude oil seep transport to the Strait of Magellan in southern Patagonia.

The Science of the total environment·2024
Same author

Global taxonomic, functional, and phylogenetic diversity of bees in apple orchards.

The Science of the total environment·2023
Same author

Effects of bisphenol F, bisphenol S, and bisphenol AF on cultured human osteoblasts.

Archives of toxicology·2023
Same author

Direct Measurement of Resonances in ^{7}Be(α,γ)^{11}C Relevant to νp-Process Nucleosynthesis.

Physical review letters·2022
Same author

High accuracy astigmatic-focusing system for laser targets.

Applied optics·2022
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Jul 22, 2025

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

15.0K

Nonlinear confocal positioner for micron-scale target alignment.

J Delgado-Aguillón, C Ruiz, M Rosete-Aguilar

    Optics Express
    |July 21, 2023
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a novel target positioner system with micron-scale precision. Its nonlinear detection scheme enhances sensitivity for applications in laser microfabrication and plasma accelerators.

    More Related Videos

    Automated Delivery of Microfabricated Targets for Intense Laser Irradiation Experiments
    06:40

    Automated Delivery of Microfabricated Targets for Intense Laser Irradiation Experiments

    Published on: January 28, 2021

    4.4K
    Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces
    08:05

    Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces

    Published on: September 9, 2022

    2.4K

    Related Experiment Videos

    Last Updated: Jul 22, 2025

    A Protocol for Real-time 3D Single Particle Tracking
    10:16

    A Protocol for Real-time 3D Single Particle Tracking

    Published on: January 3, 2018

    15.0K
    Automated Delivery of Microfabricated Targets for Intense Laser Irradiation Experiments
    06:40

    Automated Delivery of Microfabricated Targets for Intense Laser Irradiation Experiments

    Published on: January 28, 2021

    4.4K
    Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces
    08:05

    Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces

    Published on: September 9, 2022

    2.4K

    Area of Science:

    • Optics and Photonics
    • Precision Engineering
    • Laser Technology

    Background:

    • Accurate target positioning is crucial for advanced scientific applications.
    • Existing systems often face limitations in sensitivity, accuracy, or alignment complexity.

    Purpose of the Study:

    • To present a novel target positioner system with high sensitivity and accuracy.
    • To demonstrate a system feasible for microfabrication and laser-driven plasma accelerators.

    Main Methods:

    • Utilized a nonlinear detection scheme with two-photon-absorption in a Si-photodiode and CMOS sensor at 1550 nm.
    • Employed a confocal configuration for ease of alignment and tilt tolerance, eliminating the need for a pinhole aperture.

    Main Results:

    • Achieved micron-scale precision in locating rough target surfaces within the focal plane.
    • Demonstrated high accuracy up to 5 μm, limited by focus scanning step size.
    • System exhibits high sensitivity due to the nonlinear detection process.

    Conclusions:

    • The novel target positioner system offers significant advancements in precision and sensitivity.
    • Potential applications include laser microfabrication and high-repetition-rate laser-driven plasma accelerators.
    • The system's design is adaptable for cameras and other spectral ranges.