Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

654
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
654
Gauss's Law: Problem-Solving01:10

Gauss's Law: Problem-Solving

1.8K
Gauss's law helps determine electric fields even though the law is not directly about electric fields but electric flux. In situations with certain symmetries (spherical, cylindrical, or planar) in the charge distribution, the electric field can be deduced based on the knowledge of the electric flux. In these systems, we can find a Gaussian surface S over which the electric field has a constant magnitude. Furthermore, suppose the electric field is parallel (or antiparallel) to the area...
1.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An open-source replication for fast and accessible light propagation modeling in brain tissue.

PloS one·2025
Same author

Robust and practical measurement of volume transport parameters in solid photo-polymer materials for 3D printing.

Optics express·2021
Same author

Virtual ellipsometry on layered micro-facet surfaces.

Optics express·2017
Same author

Modeling and verifying the polarizing reflectance of real-world metallic surfaces.

IEEE computer graphics and applications·2014
Same author

Adding a solar-radiance function to the Hošek-Wilkie skylight model.

IEEE computer graphics and applications·2014

Related Experiment Video

Updated: Jul 22, 2025

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
11:22

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions

Published on: January 30, 2018

10.1K

Affordable method for measuring fluorescence using Gaussian distributions and bounded MESE.

Tomáš Iser, Loïc Lachiver, Alexander Wilkie

    Optics Express
    |July 21, 2023
    PubMed
    Summary

    We developed a new, affordable method to measure material fluorescence using spectral characterization. This technique requires minimal reflectance measurements, offering accurate Donaldson matrix estimates with improved robustness and lower error rates compared to existing methods.

    More Related Videos

    A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
    08:43

    A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

    Published on: December 1, 2018

    11.4K
    Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System
    12:30

    Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System

    Published on: February 9, 2017

    12.2K

    Related Experiment Videos

    Last Updated: Jul 22, 2025

    Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
    11:22

    Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions

    Published on: January 30, 2018

    10.1K
    A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
    08:43

    A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

    Published on: December 1, 2018

    11.4K
    Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System
    12:30

    Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System

    Published on: February 9, 2017

    12.2K

    Area of Science:

    • Optics and Photonics
    • Materials Science
    • Computational Imaging

    Background:

    • Accurate spectral characterization of material fluorescence and reflectance is crucial for various applications.
    • Existing methods for estimating Donaldson matrices can be costly, complex, or prone to artifacts.
    • There is a need for low-cost, robust, and accurate techniques for spectral measurements.

    Purpose of the Study:

    • To present an accurate and low-cost method for measuring material fluorescence.
    • To estimate the Donaldson matrix, a key spectral characterization, using minimal input data.
    • To demonstrate the method's effectiveness with readily available optical components.

    Main Methods:

    • Utilizing a multivariate Gaussian mixture model to represent Donaldson matrices.
    • Employing a bounded Maximum Entropy Spectral Estimate (MESE) for spectral estimation.
    • Implementing gradient-descent optimization with robust parametrization and constraints.

    Main Results:

    • Achieved significantly lower estimation errors compared to the state-of-the-art on identical datasets.
    • Demonstrated robustness against spectral artifacts like oscillations.
    • Validated the method on both synthetic and real-world data, including diverse optical components.

    Conclusions:

    • The proposed method offers a cost-effective and accurate approach to spectral characterization of fluorescence and reflectance.
    • The algorithm's robustness and stability make it a reliable tool for materials analysis.
    • This technique advances the field by providing a more accessible and precise way to measure optical properties.